Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS25775 and BPHYT_RS25780 are separated by 536 nucleotidesBPHYT_RS25780 and BPHYT_RS25785 are separated by 65 nucleotidesBPHYT_RS25785 and BPHYT_RS25790 are separated by 15 nucleotidesBPHYT_RS25790 and BPHYT_RS25795 are separated by 146 nucleotides BPHYT_RS25775: BPHYT_RS25775 - hypothetical protein, at 1,355,473 to 1,355,751 _RS25775 BPHYT_RS25780: BPHYT_RS25780 - DeoR faimly transcriptional regulator, at 1,356,288 to 1,357,013 _RS25780 BPHYT_RS25785: BPHYT_RS25785 - sugar ABC transporter permease, at 1,357,079 to 1,358,137 _RS25785 BPHYT_RS25790: BPHYT_RS25790 - sugar ABC transporter ATP-binding protein, at 1,358,153 to 1,359,655 _RS25790 BPHYT_RS25795: BPHYT_RS25795 - LacI family transcription regulator, at 1,359,802 to 1,360,806 _RS25795
Group Condition BPHYT_RS25775 BPHYT_RS25780 BPHYT_RS25785 BPHYT_RS25790 BPHYT_RS25795
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. -4.3 -0.0 N.D. N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. -3.7 -0.5 N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -4.2 +0.4 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. -3.7 +0.0 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days N.D. -3.5 +0.3 N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -2.9 -0.2 N.D. N.D.
carbon source L-Arginine (C) N.D. -2.2 -0.5 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -2.7 +0.0 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -2.5 -0.0 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -2.4 -0.0 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. -2.7 +0.3 N.D. N.D.
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. -0.1 -2.2 N.D. N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. -1.7 -0.6 N.D. N.D.
carbon source Phenylacetic 5 mM (C) N.D. -2.1 +0.3 N.D. N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. -2.2 +0.5 N.D. N.D.
carbon source Sebacic (C) N.D. -2.0 +0.6 N.D. N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X N.D. -1.9 +0.7 N.D. N.D.
phage 24hr PHYTO1 MOI 1 N.D. -1.8 +0.6 N.D. N.D.
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. -2.0 +0.9 N.D. N.D.
phage 24hr PHYTO10 MOI 1 N.D. -1.6 +0.7 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +2.1 -0.6 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +2.3 -0.4 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +1.3 +1.0 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +2.4 +0.2 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +2.9 -0.0 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +3.4 +0.1 N.D. N.D.
carbon source 2-Deoxy-D-Ribose (C) N.D. +1.9 +2.8 N.D. N.D.
carbon source 2-Deoxy-D-Ribose (C) N.D. +2.6 +2.6 N.D. N.D.
carbon source 2-Deoxy-D-Ribose (C) N.D. +1.4 +4.0 N.D. N.D.
carbon source 2-Deoxy-D-Ribose (C) N.D. +1.5 +4.3 N.D. N.D.
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