Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS25565 and BPHYT_RS25570 are separated by 272 nucleotidesBPHYT_RS25570 and BPHYT_RS25575 are separated by 495 nucleotidesBPHYT_RS25575 and BPHYT_RS25580 are separated by 93 nucleotidesBPHYT_RS25580 and BPHYT_RS25585 are separated by 258 nucleotides BPHYT_RS25565: BPHYT_RS25565 - hypothetical protein, at 1,308,230 to 1,308,871 _RS25565 BPHYT_RS25570: BPHYT_RS25570 - hypothetical protein, at 1,309,144 to 1,309,389 _RS25570 BPHYT_RS25575: BPHYT_RS25575 - alpha/beta hydrolase, at 1,309,885 to 1,310,751 _RS25575 BPHYT_RS25580: BPHYT_RS25580 - membrane protein, at 1,310,845 to 1,311,318 _RS25580 BPHYT_RS25585: BPHYT_RS25585 - membrane protein, at 1,311,577 to 1,311,888 _RS25585
Group Condition BPHYT_RS25565 BPHYT_RS25570 BPHYT_RS25575 BPHYT_RS25580 BPHYT_RS25585
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 N.D. -5.3 N.D. -0.1
phage 24hr PHYTO1 MOI 1 -0.6 N.D. -2.5 N.D. +0.4
phage 24hr PHYTO6 MOI 10 -0.3 N.D. -1.8 N.D. -0.3
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.8 N.D. -1.1 N.D. -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 N.D. -1.2 N.D. -0.9
phage P. phyto PHYTO2 MOI 1 +0.3 N.D. -0.5 N.D. -1.5
phage 36hr PHYTO11 MOI 0.1 -1.1 N.D. +0.3 N.D. -0.8
phage 36hr PHYTO3 MOI 1 -0.3 N.D. +0.4 N.D. -1.6
carbon source Tween 20 (C) +0.1 N.D. +0.4 N.D. -1.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.8 N.D. -0.4 N.D. -1.5
carbon source Phenylacetic 5 mM (C) +0.0 N.D. -2.6 N.D. +1.6
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.6 N.D. +0.1 N.D. -1.5
vitamin No vitamins t1 -0.9 N.D. +0.9 N.D. -0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 N.D. +0.8 N.D. -1.6
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.4 N.D. +1.0 N.D. -1.0
vitamin With vitamins t1 -0.6 N.D. +1.0 N.D. -0.7
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.8 N.D. -2.6 N.D. +1.6
phage 24hr PHYTO MOI 0.1 +1.1 N.D. -0.3 N.D. -0.9
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.8 N.D. +1.7 N.D. -0.7
phage PHYTO3 MOI 0.1 +0.2 N.D. -0.9 N.D. +1.2
nitrogen source L-Lysine (N) +0.6 N.D. +0.9 N.D. -1.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 N.D. +1.6 N.D. -0.5
phage P. phyto PHYTO2 MOI 1 +0.2 N.D. +1.2 N.D. -0.7
phage 36hr PHYTO3 MOI 1 +1.5 N.D. -0.6 N.D. +0.1
phage 24hr PHYTO3 MOI 10 +0.6 N.D. +1.8 N.D. -0.6
phage 24hr PHYTO3 MOI 10 -0.1 N.D. +2.1 N.D. +0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +1.1 N.D. +0.5 N.D. +0.6
carbon source Disodium Glutarate (C) +0.1 N.D. +1.8 N.D. +0.4
phage 36hr PHYTO6 MOI 10 +0.8 N.D. +1.6 N.D. +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.3 N.D. +1.7 N.D. +0.6
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BPHYT_RS25570
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