Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS25550 and BPHYT_RS25555 are separated by 272 nucleotidesBPHYT_RS25555 and BPHYT_RS25560 are separated by 100 nucleotidesBPHYT_RS25560 and BPHYT_RS25565 overlap by 4 nucleotidesBPHYT_RS25565 and BPHYT_RS25570 are separated by 272 nucleotides BPHYT_RS25550: BPHYT_RS25550 - hypothetical protein, at 1,305,915 to 1,306,607 _RS25550 BPHYT_RS25555: BPHYT_RS25555 - alkyl hydroperoxide reductase, at 1,306,880 to 1,307,560 _RS25555 BPHYT_RS25560: BPHYT_RS25560 - RNA polymerase sigma factor, at 1,307,661 to 1,308,233 _RS25560 BPHYT_RS25565: BPHYT_RS25565 - hypothetical protein, at 1,308,230 to 1,308,871 _RS25565 BPHYT_RS25570: BPHYT_RS25570 - hypothetical protein, at 1,309,144 to 1,309,389 _RS25570
Group Condition BPHYT_RS25550 BPHYT_RS25555 BPHYT_RS25560 BPHYT_RS25565 BPHYT_RS25570
nitrogen source L-Isoleucine (N) N.D. N.D. -1.9 -0.7 N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. N.D. -1.0 -0.8 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. N.D. -0.8 -0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -1.3 -0.4 N.D.
carbon source Phenylacetic 5 mM (C) N.D. N.D. -1.3 -0.3 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. N.D. -1.4 -0.2 N.D.
nitrogen source Ammonium chloride (N) N.D. N.D. -1.2 -0.3 N.D.
temperature LB, 37C N.D. N.D. -0.9 -0.6 N.D.
lb_dap plate control LB_DAP agar plate, outgrowth in LB N.D. N.D. -0.5 -1.0 N.D.
nitrogen source L-Glutamic (N) N.D. N.D. -1.4 -0.1 N.D.
phage 36hr PHYTO11 MOI 1 N.D. N.D. -1.7 +0.5 N.D.
phage 36hr PHYTO10 MOI 10 N.D. N.D. -1.4 +0.4 N.D.
phage 36hr PHYTO11 MOI 0.1 N.D. N.D. +0.6 -1.1 N.D.
phage 36hr PHYTO2 MOI 1 N.D. N.D. -1.0 +0.6 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. +0.8 -0.8 N.D.
vitamin With vitamins t2 N.D. N.D. +0.9 -0.8 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +1.3 -1.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. +1.4 -1.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. +1.3 -0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +1.0 +0.6 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.9 +0.7 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. +1.2 +0.4 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. N.D. +1.0 +0.7 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. N.D. +0.6 +1.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days N.D. N.D. +1.5 +0.2 N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. N.D. +1.7 +0.1 N.D.
phage 36hr PHYTO3 MOI 1 N.D. N.D. +0.3 +1.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +1.1 +0.8 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.5 +1.6 N.D.
carbon source Malonic (C) N.D. N.D. +1.0 +1.4 N.D.
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