Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS25485 and BPHYT_RS25490 overlap by 11 nucleotidesBPHYT_RS25490 and BPHYT_RS25495 are separated by 32 nucleotidesBPHYT_RS25495 and BPHYT_RS25500 are separated by 158 nucleotidesBPHYT_RS25500 and BPHYT_RS25505 overlap by 4 nucleotides BPHYT_RS25485: BPHYT_RS25485 - hypothetical protein, at 1,294,971 to 1,295,750 _RS25485 BPHYT_RS25490: BPHYT_RS25490 - hypothetical protein, at 1,295,740 to 1,296,663 _RS25490 BPHYT_RS25495: BPHYT_RS25495 - membrane protein, at 1,296,696 to 1,297,007 _RS25495 BPHYT_RS25500: BPHYT_RS25500 - hypothetical protein, at 1,297,166 to 1,297,807 _RS25500 BPHYT_RS25505: BPHYT_RS25505 - RNA polymerase sigma factor, at 1,297,804 to 1,298,349 _RS25505
Group Condition BPHYT_RS25485 BPHYT_RS25490 BPHYT_RS25495 BPHYT_RS25500 BPHYT_RS25505
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. +0.6 N.D. -4.8 -0.3
carbon source Tween 20 (C) N.D. -0.1 N.D. -4.7 +0.4
phage 36hr PHYTO1 MOI 1 N.D. -0.9 N.D. -2.5 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.1 N.D. -4.0 +0.3
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. +0.4 N.D. -3.1 -0.8
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.6 N.D. -2.6 +0.1
phage 36hr PHYTO8 MOI 0.1 N.D. -0.7 N.D. -2.5 +0.0
nitrogen source L-Isoleucine (N) N.D. -0.9 N.D. -2.4 +0.2
phage 24hr PHYTO11 MOI 0.1 N.D. -0.6 N.D. -2.5 +0.4
phage 36hr PHYTO9 MOI 0.1 N.D. -0.4 N.D. -1.7 -0.5
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. +0.4 N.D. -2.7 -0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. -1.2 N.D. -0.8 -0.5
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +0.2 N.D. -2.0 -0.5
phage P. phyto PHYTO2 MOI 1 N.D. +0.2 N.D. -2.5 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.6 N.D. -2.6 -0.3
phage 36hr PHYTO6 MOI 1 N.D. +0.3 N.D. -2.3 -0.1
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. -0.4 N.D. -1.8 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. -0.1 N.D. -2.4 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.2 N.D. -2.3 +0.7
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. +0.4 N.D. -1.8 -0.3
phage 36hr PHYTO2 MOI 0.1 N.D. +1.0 N.D. -2.7 +0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.5 N.D. -2.1 +0.0
phage P. phyto corn MOI 1 N.D. +0.2 N.D. -2.3 +0.5
phage 36hr PHYTO1 MOI 10 N.D. +0.7 N.D. -1.6 -0.4
carbon source 2-Deoxy-D-Ribose (C) N.D. -0.0 N.D. -1.7 +0.7
phage 24hr PHYTO6 MOI 0.1 N.D. +1.0 N.D. -1.7 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.1 N.D. -1.6 +0.7
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. -0.4 N.D. +1.8 -0.3
nophagecontrol 24hr Phyto Lib only N.D. -0.3 N.D. +1.9 -0.2
phage 36hr PHYTO11 MOI 1 N.D. +1.0 N.D. +1.5 -0.0
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BPHYT_RS25485
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BPHYT_RS25490
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