Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS25465 and BPHYT_RS25470 are separated by 26 nucleotidesBPHYT_RS25470 and BPHYT_RS25475 are separated by 115 nucleotidesBPHYT_RS25475 and BPHYT_RS25480 are separated by 173 nucleotidesBPHYT_RS25480 and BPHYT_RS25485 are separated by 91 nucleotides BPHYT_RS25465: BPHYT_RS25465 - 1,2-oxophytodienoate reductase, at 1,291,383 to 1,292,471 _RS25465 BPHYT_RS25470: BPHYT_RS25470 - alkylhydroperoxidase, at 1,292,498 to 1,293,034 _RS25470 BPHYT_RS25475: BPHYT_RS25475 - LysR family transcriptional regulator, at 1,293,150 to 1,294,064 _RS25475 BPHYT_RS25480: BPHYT_RS25480 - hypothetical protein, at 1,294,238 to 1,294,879 _RS25480 BPHYT_RS25485: BPHYT_RS25485 - hypothetical protein, at 1,294,971 to 1,295,750 _RS25485
Group Condition BPHYT_RS25465 BPHYT_RS25470 BPHYT_RS25475 BPHYT_RS25480 BPHYT_RS25485
phage P. phyto PHYTO2 MOI 1 +0.2 -2.7 N.D. -1.5 N.D.
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.1 -2.9 N.D. -0.0 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.4 -2.9 N.D. -0.2 N.D.
phage P. phyto corn MOI 0.1 -0.5 -1.7 N.D. -0.5 N.D.
phage 24hr PHYTO7 MOI 10 -0.2 -0.6 N.D. -1.6 N.D.
phage 24hr PHYTO11 MOI 1 -0.2 -0.1 N.D. -2.0 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -2.1 N.D. -0.3 N.D.
phage 24hr PHYTO3 MOI 10 -1.0 -1.1 N.D. -0.2 N.D.
phage P. phyto corn MOI 1 -0.3 -1.3 N.D. -0.5 N.D.
phage 24hr PHYTO3 MOI 10 -0.3 -0.7 N.D. -1.1 N.D.
vitamin No vitamins t1 +0.3 -1.4 N.D. -0.7 N.D.
nophagecontrol 36hr Phyto Lib only +0.3 -1.8 N.D. -0.2 N.D.
phage 36hr PHYTO1 MOI 10 +0.3 -1.9 N.D. +0.0 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 -1.6 N.D. -0.4 N.D.
phage 24hr PHYTO7 MOI 1 +0.5 -1.5 N.D. -0.5 N.D.
phage 24hr PHYTO2 MOI 0.1 +0.4 -1.9 N.D. +0.5 N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.6 -1.5 N.D. +0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.1 -1.7 N.D. +1.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 -1.4 N.D. +0.8 N.D.
phage 24hr PHYTO4 MOI 10 +0.1 +0.9 N.D. -1.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.5 -1.3 N.D. +0.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 -1.2 N.D. +0.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -1.5 N.D. +0.8 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 -0.9 N.D. +1.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.0 -1.6 N.D. +1.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.1 +1.9 N.D. -0.9 N.D.
motility outer cut, LB soft agar motility assay +0.1 +1.8 N.D. -0.4 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.6 +0.7 N.D. +0.8 N.D.
phage 36hr PHYTO9 MOI 1 +0.8 +1.4 N.D. +0.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.9 +1.6 N.D. +1.0 N.D.
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