Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS25420 and BPHYT_RS25425 are separated by 16 nucleotidesBPHYT_RS25425 and BPHYT_RS25430 are separated by 18 nucleotidesBPHYT_RS25430 and BPHYT_RS25435 are separated by 1114 nucleotidesBPHYT_RS25435 and BPHYT_RS25440 are separated by 285 nucleotides BPHYT_RS25420: BPHYT_RS25420 - hypothetical protein, at 1,280,648 to 1,280,842 _RS25420 BPHYT_RS25425: BPHYT_RS25425 - aldehyde-activating protein, at 1,280,859 to 1,281,434 _RS25425 BPHYT_RS25430: BPHYT_RS25430 - S-(hydroxymethyl)glutathione dehydrogenase, at 1,281,453 to 1,282,562 _RS25430 BPHYT_RS25435: BPHYT_RS25435 - hypothetical protein, at 1,283,677 to 1,283,910 _RS25435 BPHYT_RS25440: BPHYT_RS25440 - alanine dehydrogenase, at 1,284,196 to 1,285,320 _RS25440
Group Condition BPHYT_RS25420 BPHYT_RS25425 BPHYT_RS25430 BPHYT_RS25435 BPHYT_RS25440
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. N.D. N.D. N.D. -1.3
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. N.D. N.D. -1.3
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. N.D. N.D. N.D. -1.3
phage 36hr PHYTO11 MOI 1 N.D. N.D. N.D. N.D. -1.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. N.D. -1.1
phage 24hr PHYTO9 MOI 10 N.D. N.D. N.D. N.D. -1.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. N.D. -1.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. N.D. -1.0
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. N.D. -1.0
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) N.D. N.D. N.D. N.D. -0.9
phage 36hr PHYTO11 MOI 0.1 N.D. N.D. N.D. N.D. -0.9
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. N.D. -0.9
motility outer cut, LB soft agar motility assay N.D. N.D. N.D. N.D. -0.8
carbon source D-Mannose (C) N.D. N.D. N.D. N.D. -0.8
temperature LB, 37C N.D. N.D. N.D. N.D. -0.8
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. N.D. N.D. N.D. +0.8
phage 36hr PHYTO9 MOI 0.1 N.D. N.D. N.D. N.D. +0.8
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. N.D. N.D. N.D. +0.8
vitamin With vitamins t2 N.D. N.D. N.D. N.D. +0.8
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. N.D. +0.8
vitamin No vitamins t1 N.D. N.D. N.D. N.D. +0.8
vitamin No vitamins t2 N.D. N.D. N.D. N.D. +0.8
phage 24hr PHYTO3 MOI 10 N.D. N.D. N.D. N.D. +0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. N.D. N.D. +0.8
carbon source Suberic (C) N.D. N.D. N.D. N.D. +0.9
carbon source Tween 20 (C) N.D. N.D. N.D. N.D. +0.9
carbon source D-Glucose (C) N.D. N.D. N.D. N.D. +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. N.D. N.D. +1.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. N.D. +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. N.D. +4.0
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