Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS25220 and BPHYT_RS25225 are separated by 136 nucleotidesBPHYT_RS25225 and BPHYT_RS25230 are separated by 96 nucleotidesBPHYT_RS25230 and BPHYT_RS25235 are separated by 138 nucleotidesBPHYT_RS25235 and BPHYT_RS25240 are separated by 163 nucleotides BPHYT_RS25220: BPHYT_RS25220 - ferredoxin oxidoreductase, at 1,232,139 to 1,233,386 _RS25220 BPHYT_RS25225: BPHYT_RS25225 - glycine/betaine ABC transporter substrate-binding protein, at 1,233,523 to 1,234,380 _RS25225 BPHYT_RS25230: BPHYT_RS25230 - formyltetrahydrofolate deformylase, at 1,234,477 to 1,235,352 _RS25230 BPHYT_RS25235: BPHYT_RS25235 - LysR family transcriptional regulator, at 1,235,491 to 1,236,405 _RS25235 BPHYT_RS25240: BPHYT_RS25240 - choline-sulfatase, at 1,236,569 to 1,238,107 _RS25240
Group Condition BPHYT_RS25220 BPHYT_RS25225 BPHYT_RS25230 BPHYT_RS25235 BPHYT_RS25240
nitrogen source Carnitine Hydrochloride (N) -5.0 N.D. -0.3 -0.4 +0.6
phage 36hr PHYTO8 MOI 10 -1.2 N.D. -0.8 -0.0 -1.1
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -2.9 N.D. +0.0 -0.0 +0.1
phage 24hr PHYTO10 MOI 10 -1.9 N.D. -0.6 +0.0 -0.3
nophagecontrol 36hr Phyto Lib Only -2.1 N.D. +0.0 +0.1 -0.6
phage 36hr PHYTO4 MOI 1 -0.9 N.D. -0.1 -0.8 -0.7
phage 24hr PHYTO7 MOI 1 -0.6 N.D. -1.0 -0.2 -0.6
phage 36hr PHYTO3 MOI 1 -1.5 N.D. +0.1 -0.7 -0.2
phage 24hr PHYTO2 MOI 0.1 -1.3 N.D. -0.1 -0.5 -0.4
nophagecontrol 24hr Phyto Lib Only -1.4 N.D. +0.1 -0.4 -0.5
phage 24hr PHYTO MOI 0.1 -1.5 N.D. -1.2 +0.4 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days -1.4 N.D. +0.2 -0.5 -0.4
phage 24hr PHYTO11 MOI 0.1 -0.7 N.D. +0.0 +0.6 -1.2
phage 36hr PHYTO4 MOI 1 -1.6 N.D. +0.2 +0.4 -0.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -1.4 N.D. -0.8 +0.5 +0.4
phage 24hr PHYTO6 MOI 10 -1.7 N.D. -0.2 +0.7 -0.1
phage 24hr PHYTO11 MOI 1 -1.3 N.D. +0.6 +0.4 -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 N.D. -1.3 -0.1 +0.3
phage 36hr PHYTO6 MOI 1 +0.4 N.D. -1.2 +0.8 -0.6
supernatant control Vogels_fungal_media; pH 5.5 -0.3 N.D. +0.2 -1.1 +0.9
carbon source Phenylacetic 5 mM (C) -0.7 N.D. -0.1 +1.1 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +1.2 N.D. -0.2 -0.5 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.8 N.D. -0.2 +1.0 +0.2
motility outer cut, LB soft agar motility assay +0.5 N.D. -0.7 +1.3 +0.0
phage P. phyto PHYTO2 MOI 1 +1.3 N.D. +0.0 +0.7 -0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.6 N.D. +0.7 +0.9 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 N.D. -0.0 -0.2 +2.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.9 N.D. +0.6 +0.1 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 N.D. -0.0 +1.0 +1.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.6 N.D. +0.9 +0.7 +0.9
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