Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS25195 and BPHYT_RS25200 are separated by 2 nucleotidesBPHYT_RS25200 and BPHYT_RS25205 are separated by 4 nucleotidesBPHYT_RS25205 and BPHYT_RS25210 overlap by 4 nucleotidesBPHYT_RS25210 and BPHYT_RS25215 are separated by 73 nucleotides BPHYT_RS25195: BPHYT_RS25195 - N-methylproline demethylase, at 1,224,780 to 1,226,843 _RS25195 BPHYT_RS25200: BPHYT_RS25200 - (Fe-S)-binding protein, at 1,226,846 to 1,228,759 _RS25200 BPHYT_RS25205: BPHYT_RS25205 - electron transfer flavoprotein subunit alpha, at 1,228,764 to 1,229,945 _RS25205 BPHYT_RS25210: BPHYT_RS25210 - drug:proton antiporter, at 1,229,942 to 1,230,718 _RS25210 BPHYT_RS25215: BPHYT_RS25215 - (Fe-S)-binding protein, at 1,230,792 to 1,232,105 _RS25215
Group Condition BPHYT_RS25195 BPHYT_RS25200 BPHYT_RS25205 BPHYT_RS25210 BPHYT_RS25215
nitrogen source Carnitine Hydrochloride (N) N.D. -2.3 -3.9 -3.5 N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. -1.8 -3.4 -0.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. -1.3 -0.9 -0.8 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days N.D. -1.4 -1.1 -0.5 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. -0.6 -0.6 -1.8 N.D.
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.0 -1.2 -0.4 N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. -0.2 -0.5 -1.5 N.D.
carbon source Suberic (C) N.D. +0.0 -0.0 -2.2 N.D.
carbon source L-Fucose (C) N.D. -0.2 -0.1 -1.8 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -1.0 +0.4 -1.4 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. -0.3 -0.5 -1.2 N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. -0.3 -0.1 -1.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.4 -0.1 -1.3 N.D.
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.9 -0.9 +0.1 N.D.
phage 24hr PHYTO4 MOI 10 N.D. +0.3 +0.2 -2.1 N.D.
carbon source 2-Deoxy-D-Ribose (C) N.D. -0.5 +0.2 -1.1 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. -1.2 -0.4 +0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.4 +0.1 -1.8 N.D.
phage 36hr PHYTO9 MOI 1 N.D. +0.3 +0.1 -1.8 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days N.D. -0.3 -0.8 +1.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.7 -0.1 +1.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.6 +0.2 +1.6 N.D.
phage 24hr PHYTO3 MOI 10 N.D. +0.8 -0.3 +0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.2 +0.3 +1.3 N.D.
phage 24hr PHYTO1 MOI 10 N.D. +0.6 -0.3 +1.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.3 +0.5 +1.5 N.D.
phage 24hr PHYTO MOI 0.1 N.D. +0.6 +0.2 +1.0 N.D.
phage 36hr PHYTO2 MOI 1 N.D. -0.1 +0.1 +1.8 N.D.
nophagecontrol 24hr Phyto Lib Only N.D. +0.3 +0.1 +1.5 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +0.5 +0.3 +1.7 N.D.
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BPHYT_RS25215
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