Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS25145 and BPHYT_RS25150 are separated by 17 nucleotidesBPHYT_RS25150 and BPHYT_RS25155 are separated by 154 nucleotidesBPHYT_RS25155 and BPHYT_RS25160 are separated by 11 nucleotidesBPHYT_RS25160 and BPHYT_RS25165 are separated by 42 nucleotides BPHYT_RS25145: BPHYT_RS25145 - cupin, at 1,213,285 to 1,213,686 _RS25145 BPHYT_RS25150: BPHYT_RS25150 - class C beta-lactamase, at 1,213,704 to 1,214,870 _RS25150 BPHYT_RS25155: BPHYT_RS25155 - oxygen-dependent choline dehydrogenase, at 1,215,025 to 1,216,710 _RS25155 BPHYT_RS25160: BPHYT_RS25160 - NAD/NADP-dependent betaine aldehyde dehydrogenase, at 1,216,722 to 1,218,191 _RS25160 BPHYT_RS25165: BPHYT_RS25165 - HTH-type transcriptional regulator BetI, at 1,218,234 to 1,218,821 _RS25165
Group Condition BPHYT_RS25145 BPHYT_RS25150 BPHYT_RS25155 BPHYT_RS25160 BPHYT_RS25165
motility inner cut, LB soft agar motility assay -0.5 +0.1 -0.5 -1.0 -0.2
motility outer cut, LB soft agar motility assay -0.5 -0.5 +0.1 -0.1 -1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.8 -0.4 +0.6 -0.3 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.3 +0.1 +0.2 -1.8 +0.1
phage 36hr PHYTO9 MOI 0.1 +0.4 -0.4 -0.1 -0.1 -1.4
carbon source adipate (C) +0.4 -0.9 +0.1 -0.7 -0.2
phage P. phyto PHYTO2 MOI 1 +0.3 -0.4 -0.0 -1.7 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.4 -0.2 -0.1 -0.6 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 -0.4 +0.5 -1.0 +0.0
nophagecontrol 36hr Phyto Lib only +0.5 +0.3 +0.1 -0.3 -1.6
phage 24hr PHYTO6 MOI 1 -0.8 -0.5 +0.2 -0.5 +0.8
phage 36hr PHYTO1 MOI 10 +0.2 +0.1 +0.4 +0.1 -1.5
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -1.1 +0.3 -0.4 +0.1 +0.4
nitrogen source NAG (N) +0.6 -0.3 +0.2 -0.7 -0.5
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.6 -0.6 +0.1 +0.2 +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.2 +0.9 +0.3 -1.2
phage 36hr PHYTO2 MOI 1 -0.4 +0.0 +0.7 -0.8 +0.6
phage 24hr PHYTO10 MOI 1 -0.2 +0.3 +0.2 -0.7 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.4 -0.5 +0.2 +0.0 +1.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 +0.4 +0.2 +0.0 +0.9
phage 36hr PHYTO4 MOI 1 +0.1 -0.3 +0.5 -0.6 +1.0
phage 24hr PHYTO9 MOI 0.1 +0.6 +0.2 +0.2 -0.7 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.1 +0.5 -0.5 +0.1 +1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 +0.5 +0.1 -0.7 +1.1
carbon source Suberic (C) +0.3 -0.1 +0.6 +0.7 +0.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.5 +0.6 -0.4 +0.4 +1.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.5 +0.6 -0.5 +0.3 +1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.1 +0.1 +0.1 +0.3 +0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.9 +1.4 +0.1 -0.1 +0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.8 +0.3 -0.4 +0.6 +1.6
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