Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS24680 and BPHYT_RS24685 are separated by 242 nucleotidesBPHYT_RS24685 and BPHYT_RS24690 are separated by 54 nucleotidesBPHYT_RS24690 and BPHYT_RS24695 are separated by 477 nucleotidesBPHYT_RS24695 and BPHYT_RS24700 are separated by 417 nucleotides BPHYT_RS24680: BPHYT_RS24680 - hypothetical protein, at 1,106,437 to 1,106,712 _RS24680 BPHYT_RS24685: BPHYT_RS24685 - LysR family transcriptional regulator, at 1,106,955 to 1,107,848 _RS24685 BPHYT_RS24690: BPHYT_RS24690 - ionic transporter y4hA, at 1,107,903 to 1,108,985 _RS24690 BPHYT_RS24695: BPHYT_RS24695 - hypothetical protein, at 1,109,463 to 1,109,810 _RS24695 BPHYT_RS24700: BPHYT_RS24700 - 3-methyladenine DNA glycosylase, at 1,110,228 to 1,110,869 _RS24700
Group Condition BPHYT_RS24680 BPHYT_RS24685 BPHYT_RS24690 BPHYT_RS24695 BPHYT_RS24700
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -0.7 -0.7 -0.4 N.D. -0.3
carbon source D-Glucose (C) -0.9 -0.8 +0.2 N.D. -0.1
nitrogen source L-Phenylalanine (N) -1.0 -0.4 -0.1 N.D. +0.1
carbon source D-Glucose 20 mM (C) -0.6 -0.9 -0.2 N.D. +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.8 +0.2 -0.6 N.D. -0.1
nitrogen source NAG (N) -0.5 -1.2 +0.2 N.D. +0.2
nutrient Min media with glucose -0.5 -0.8 +0.3 N.D. -0.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.4 -0.9 -0.2 N.D. +0.4
phage 24hr PHYTO3 MOI 10 -0.7 +0.2 -0.7 N.D. +0.1
nitrogen source L-Citrulline (N) -0.4 -1.1 +0.3 N.D. +0.3
carbon source D-Glucose 20 mM (C) -0.6 -0.7 +0.2 N.D. +0.2
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.5 -0.9 +0.2 N.D. +0.4
nitrogen source L-Lysine (N) -1.1 -0.4 +1.3 N.D. -0.3
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -1.0 +0.2 +0.2 N.D. +0.3
phage 24hr PHYTO7 MOI 1 +0.7 +0.3 -0.9 N.D. -0.0
carbon source L-Glutamine (C) -0.5 +0.1 +0.9 N.D. -0.2
phage 24hr PHYTO9 MOI 10 +0.9 +0.3 -0.6 N.D. +0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 +0.2 +0.8 N.D. +0.4
carbon source D-Glucose (C) +1.3 -0.4 -0.1 N.D. -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 +0.2 +1.4 N.D. -0.1
motility inner cut, LB soft agar motility assay -0.6 +0.4 +1.2 N.D. -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 +0.3 +0.8 N.D. +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.6 +0.2 +0.9 N.D. -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 -0.2 +1.1 N.D. +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 +0.3 +0.8 N.D. +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.5 +0.8 N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 +0.4 +0.9 N.D. +0.3
motility outer cut, LB soft agar motility assay +0.2 +0.1 +1.4 N.D. +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 +0.7 +1.2 N.D. +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 +0.5 +2.0 N.D. +0.2
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