Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS24435 and BPHYT_RS24440 are separated by 4 nucleotidesBPHYT_RS24440 and BPHYT_RS24445 overlap by 4 nucleotidesBPHYT_RS24445 and BPHYT_RS24450 are separated by 259 nucleotidesBPHYT_RS24450 and BPHYT_RS24455 are separated by 124 nucleotides BPHYT_RS24435: BPHYT_RS24435 - cell envelope biogenesis protein OmpA, at 1,048,325 to 1,049,287 _RS24435 BPHYT_RS24440: BPHYT_RS24440 - type VI secretion protein, at 1,049,292 to 1,050,284 _RS24440 BPHYT_RS24445: BPHYT_RS24445 - type VI secretion protein VasK, at 1,050,281 to 1,054,147 _RS24445 BPHYT_RS24450: BPHYT_RS24450 - D-alanyl-D-alanine carboxypeptidase, at 1,054,407 to 1,055,252 _RS24450 BPHYT_RS24455: BPHYT_RS24455 - hypothetical protein, at 1,055,377 to 1,056,312 _RS24455
Group Condition BPHYT_RS24435 BPHYT_RS24440 BPHYT_RS24445 BPHYT_RS24450 BPHYT_RS24455
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 -0.3 -0.4 -0.3 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.2 -0.7 -0.2 -0.3 N.D.
phage 36hr PHYTO11 MOI 0.1 -0.2 -0.5 -0.2 -0.6 N.D.
phage 24hr PHYTO10 MOI 1 -0.3 -0.6 -0.4 -0.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 -0.8 -0.2 -0.1 N.D.
phage 36hr PHYTO4 MOI 1 -0.3 -0.9 -0.3 +0.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.4 -0.5 -0.0 -0.5 N.D.
phage 36hr PHYTO6 MOI 0.1 -0.3 -0.6 -0.3 -0.1 N.D.
phage 24hr PHYTO MOI 0.1 -0.4 -0.2 -0.1 -0.5 N.D.
phage 36hr PHYTO7 MOI 1 -0.0 -0.7 -0.3 -0.2 N.D.
phage 24hr PHYTO4 MOI 0.1 -0.1 -0.5 -0.3 -0.3 N.D.
phage 24hr PHYTO6 MOI 0.1 +0.1 -0.8 -0.1 -0.4 N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.1 -0.9 -0.3 +0.1 N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.1 -0.7 -0.1 -0.4 N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.0 -1.2 +0.1 +0.2 N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.0 -1.1 +0.1 +0.3 N.D.
phage 24hr PHYTO3 MOI 10 -0.2 +0.3 -0.5 -0.3 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.4 -0.7 -0.0 -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 -1.1 +0.1 +0.1 N.D.
phage 24hr PHYTO10 MOI 10 -0.6 +0.2 -0.3 +0.1 N.D.
phage 24hr PHYTO7 MOI 1 -0.3 +0.6 -0.5 -0.2 N.D.
phage 24hr PHYTO7 MOI 10 +0.2 -0.4 -0.4 +0.3 N.D.
carbon source Sebacic (C) -0.1 -0.5 -0.1 +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -0.5 +0.3 +0.3 N.D.
nitrogen source L-Glutamic (N) -0.1 +0.5 -0.4 +0.2 N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.0 -0.3 +0.2 +0.7 N.D.
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.2 +0.9 -0.0 +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 +1.0 +0.1 -0.1 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.3 +1.1 -0.0 +0.3 N.D.
nitrogen source Carnitine Hydrochloride (N) +0.4 +1.0 -0.1 +0.4 N.D.
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