Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS24335 and BPHYT_RS24340 are separated by 2 nucleotidesBPHYT_RS24340 and BPHYT_RS24345 are separated by 282 nucleotidesBPHYT_RS24345 and BPHYT_RS24350 are separated by 45 nucleotidesBPHYT_RS24350 and BPHYT_RS24355 are separated by 99 nucleotides BPHYT_RS24335: BPHYT_RS24335 - toxin HipA, at 1,025,758 to 1,027,098 _RS24335 BPHYT_RS24340: BPHYT_RS24340 - XRE family transcriptional regulator, at 1,027,101 to 1,027,355 _RS24340 BPHYT_RS24345: BPHYT_RS24345 - FAD-dependent oxidoreductase, at 1,027,638 to 1,028,978 _RS24345 BPHYT_RS24350: BPHYT_RS24350 - hypothetical protein, at 1,029,024 to 1,029,656 _RS24350 BPHYT_RS24355: BPHYT_RS24355 - hypothetical protein, at 1,029,756 to 1,030,007 _RS24355
Group Condition BPHYT_RS24335 BPHYT_RS24340 BPHYT_RS24345 BPHYT_RS24350 BPHYT_RS24355
phage 36hr PHYTO1 MOI 1 -1.2 N.D. N.D. -1.0 N.D.
phage 36hr PHYTO3 MOI 0.1 -0.6 N.D. N.D. -1.1 N.D.
phage P. phyto PHYTO2 MOI 1 +0.1 N.D. N.D. -1.8 N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.6 N.D. N.D. -1.0 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 N.D. N.D. -1.5 N.D.
nutrient L-Serine -0.0 N.D. N.D. -1.5 N.D.
phage 24hr PHYTO3 MOI 10 -0.7 N.D. N.D. -0.8 N.D.
nitrogen source L-Serine (N) +0.0 N.D. N.D. -1.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 N.D. N.D. -0.9 N.D.
phage PHYTO3 MOI 0.1 -0.7 N.D. N.D. -0.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 N.D. N.D. -1.5 N.D.
phage 36hr PHYTO3 MOI 1 +0.1 N.D. N.D. -1.3 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -1.3 N.D. N.D. +0.1 N.D.
nitrogen source L-Lysine (N) +0.8 N.D. N.D. -1.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 N.D. N.D. -1.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.7 N.D. N.D. -1.3 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.5 N.D. N.D. -0.8 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 N.D. N.D. -0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 N.D. N.D. +1.0 N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.3 N.D. N.D. +1.1 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 N.D. N.D. +1.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 N.D. N.D. +1.2 N.D.
carbon source Malonic (C) +1.0 N.D. N.D. +0.4 N.D.
carbon source Tween 20 (C) +0.3 N.D. N.D. +1.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.3 N.D. N.D. +0.3 N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.8 N.D. N.D. +1.1 N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.8 N.D. N.D. +1.2 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.8 N.D. N.D. +2.6 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +1.1 N.D. N.D. +2.7 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.9 N.D. N.D. +3.0 N.D.
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