Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS24155 and BPHYT_RS24160 are separated by 224 nucleotidesBPHYT_RS24160 and BPHYT_RS24165 are separated by 51 nucleotidesBPHYT_RS24165 and BPHYT_RS24170 overlap by 4 nucleotidesBPHYT_RS24170 and BPHYT_RS24175 are separated by 587 nucleotides BPHYT_RS24155: BPHYT_RS24155 - 5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41) (from data), at 987,186 to 988,103 _RS24155 BPHYT_RS24160: BPHYT_RS24160 - D-galactarate dehydratase, at 988,328 to 989,917 _RS24160 BPHYT_RS24165: BPHYT_RS24165 - NAD-dependent dehydratase, at 989,969 to 990,811 _RS24165 BPHYT_RS24170: BPHYT_RS24170 - gluconolactonase, at 990,808 to 991,737 _RS24170 BPHYT_RS24175: BPHYT_RS24175 - C4-dicarboxylate ABC transporter, at 992,325 to 993,317 _RS24175
Group Condition BPHYT_RS24155 BPHYT_RS24160 BPHYT_RS24165 BPHYT_RS24170 BPHYT_RS24175
carbon source D-Galacturonic Acid (C) -2.2 -1.2 -0.6 -1.1 -0.2
phage 36hr PHYTO9 MOI 1 +0.2 -0.1 -0.2 -0.2 -3.9
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 -0.0 -1.0 -0.5 -1.9
phage 24hr PHYTO1 MOI 10 +0.2 -0.0 -0.4 -0.4 -2.9
phage 24hr PHYTO2 MOI 0.1 -0.2 +0.1 -0.1 -0.1 -2.1
phage 36hr PHYTO2 MOI 1 -0.3 -0.0 -0.9 +0.1 -1.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.2 -0.1 -0.2 -1.4 -0.7
phage P. phyto PHYTO2 MOI 0.1 +0.1 -0.1 +0.1 -0.0 -2.2
nophagecontrol 24hr Phyto Lib Only -0.1 +0.0 -0.1 -0.1 -1.9
supernatant control Vogels_fungal_media; pH 5.5 +0.2 -0.4 +0.1 -0.0 -2.0
phage 36hr PHYTO11 MOI 1 +0.1 -0.3 -0.8 -1.2 +0.2
phage 36hr PHYTO10 MOI 10 -0.1 -0.1 -0.5 +0.0 -1.4
phage P. phyto PHYTO2 MOI 1 -0.0 +0.1 -0.7 +0.1 -1.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.2 -0.3 -0.7 -0.8 +0.4
phage PHYTO3 MOI 0.1 +0.3 +0.2 -0.4 +0.2 -1.7
phage 36hr PHYTO11 MOI 0.1 -0.0 +0.3 +0.2 -0.9 -0.7
phage 36hr PHYTO7 MOI 1 +0.2 +0.4 -0.3 -0.9 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 -0.5 -0.1 +0.8 -1.1
carbon source Suberic (C) -0.1 -0.7 -0.1 +0.7 -0.7
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.1 +0.0 +0.7 -0.8 -0.5
motility outer cut, LB soft agar motility assay +0.3 +0.0 +0.6 -1.1 -0.5
phage 24hr PHYTO11 MOI 0.1 +0.1 +0.2 -0.2 +0.5 -1.2
phage 36hr PHYTO8 MOI 10 +0.3 +0.0 -0.9 -0.5 +0.6
phage 36hr PHYTO1 MOI 1 -0.3 -0.1 -0.0 -0.6 +0.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.3 +0.1 -0.6 -0.3 +1.2
vitamin With vitamins t1 -0.5 -0.2 -0.2 +0.5 +0.7
carbon source Malonic (C) +0.2 +0.2 +0.5 +0.3 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 -0.6 +0.5 +0.4 +0.5
vitamin No vitamins t1 -0.1 -0.2 +0.1 +0.7 +1.0
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.1 -0.2 +0.3 +0.2 +1.3
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