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Protein
Homologs
Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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500 nt
BPHYT_RS24155 and BPHYT_RS24160 are separated by 224 nucleotides
BPHYT_RS24160 and BPHYT_RS24165 are separated by 51 nucleotides
BPHYT_RS24165 and BPHYT_RS24170 overlap by 4 nucleotides
BPHYT_RS24170 and BPHYT_RS24175 are separated by 587 nucleotides
BPHYT_RS24155: BPHYT_RS24155 - 5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41)
(from data)
, at 987,186 to 988,103
_RS24155
BPHYT_RS24160: BPHYT_RS24160 - D-galactarate dehydratase, at 988,328 to 989,917
_RS24160
BPHYT_RS24165: BPHYT_RS24165 - NAD-dependent dehydratase, at 989,969 to 990,811
_RS24165
BPHYT_RS24170: BPHYT_RS24170 - gluconolactonase, at 990,808 to 991,737
_RS24170
BPHYT_RS24175: BPHYT_RS24175 - C4-dicarboxylate ABC transporter, at 992,325 to 993,317
_RS24175
Group
Condition
BPHYT
_RS24155
BPHYT
_RS24160
BPHYT
_RS24165
BPHYT
_RS24170
BPHYT
_RS24175
carbon source
D-Galacturonic Acid (C)
-2.2
-1.2
-0.6
-1.1
-0.2
phage
36hr PHYTO9 MOI 1
+0.2
-0.1
-0.2
-0.2
-3.9
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.4
-0.0
-1.0
-0.5
-1.9
phage
24hr PHYTO1 MOI 10
+0.2
-0.0
-0.4
-0.4
-2.9
phage
24hr PHYTO2 MOI 0.1
-0.2
+0.1
-0.1
-0.1
-2.1
phage
36hr PHYTO2 MOI 1
-0.3
-0.0
-0.9
+0.1
-1.1
in planta
Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days
+0.2
-0.1
-0.2
-1.4
-0.7
phage
P. phyto PHYTO2 MOI 0.1
+0.1
-0.1
+0.1
-0.0
-2.2
nophagecontrol
24hr Phyto Lib Only
-0.1
+0.0
-0.1
-0.1
-1.9
supernatant control
Vogels_fungal_media; pH 5.5
+0.2
-0.4
+0.1
-0.0
-2.0
phage
36hr PHYTO11 MOI 1
+0.1
-0.3
-0.8
-1.2
+0.2
phage
36hr PHYTO10 MOI 10
-0.1
-0.1
-0.5
+0.0
-1.4
phage
P. phyto PHYTO2 MOI 1
-0.0
+0.1
-0.7
+0.1
-1.3
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
-0.2
-0.3
-0.7
-0.8
+0.4
phage
PHYTO3 MOI 0.1
+0.3
+0.2
-0.4
+0.2
-1.7
phage
36hr PHYTO11 MOI 0.1
-0.0
+0.3
+0.2
-0.9
-0.7
phage
36hr PHYTO7 MOI 1
+0.2
+0.4
-0.3
-0.9
-0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.0
-0.5
-0.1
+0.8
-1.1
carbon source
Suberic (C)
-0.1
-0.7
-0.1
+0.7
-0.7
in planta
Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days
-0.1
+0.0
+0.7
-0.8
-0.5
motility
outer cut, LB soft agar motility assay
+0.3
+0.0
+0.6
-1.1
-0.5
phage
24hr PHYTO11 MOI 0.1
+0.1
+0.2
-0.2
+0.5
-1.2
phage
36hr PHYTO8 MOI 10
+0.3
+0.0
-0.9
-0.5
+0.6
phage
36hr PHYTO1 MOI 1
-0.3
-0.1
-0.0
-0.6
+0.9
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB
-0.3
+0.1
-0.6
-0.3
+1.2
vitamin
With vitamins t1
-0.5
-0.2
-0.2
+0.5
+0.7
carbon source
Malonic (C)
+0.2
+0.2
+0.5
+0.3
-0.9
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.3
-0.6
+0.5
+0.4
+0.5
vitamin
No vitamins t1
-0.1
-0.2
+0.1
+0.7
+1.0
in planta
Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
+0.1
-0.2
+0.3
+0.2
+1.3
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