Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS24105 and BPHYT_RS24110 are separated by 110 nucleotidesBPHYT_RS24110 and BPHYT_RS24115 are separated by 395 nucleotidesBPHYT_RS24115 and BPHYT_RS24120 are separated by 29 nucleotidesBPHYT_RS24120 and BPHYT_RS24125 are separated by 164 nucleotides BPHYT_RS24105: BPHYT_RS24105 - chloroperoxidase, at 974,598 to 975,419 _RS24105 BPHYT_RS24110: BPHYT_RS24110 - hypothetical protein, at 975,530 to 976,072 _RS24110 BPHYT_RS24115: BPHYT_RS24115 - epimerase, at 976,468 to 977,304 _RS24115 BPHYT_RS24120: BPHYT_RS24120 - hexuronate transporter ExuT, at 977,334 to 978,626 _RS24120 BPHYT_RS24125: BPHYT_RS24125 - endopolygalacturonase, at 978,791 to 980,806 _RS24125
Group Condition BPHYT_RS24105 BPHYT_RS24110 BPHYT_RS24115 BPHYT_RS24120 BPHYT_RS24125
phage 24hr PHYTO8 MOI 1 -1.3 -0.3 -0.3 -0.6 -1.0
phage 24hr PHYTO MOI 0.1 -1.0 -0.3 -0.2 -0.9 -0.8
phage 36hr PHYTO4 MOI 1 -0.4 -0.5 -0.2 -0.8 -0.8
phage 36hr PHYTO9 MOI 0.1 -0.3 -0.5 -0.6 -0.2 -0.9
motility outer cut, LB soft agar motility assay -1.3 -0.2 -0.5 -0.4 -0.1
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -1.2 +0.3 -0.6 -0.3 -0.4
phage 36hr PHYTO1 MOI 10 +0.3 -0.1 +0.1 -1.0 -1.1
phage 24hr PHYTO2 MOI 1 +0.1 +0.4 -0.2 -1.0 -1.0
phage 24hr PHYTO3 MOI 10 -1.8 -0.3 +0.9 +0.2 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.6 -0.6 +0.6 -0.1 -0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.7 -0.2 +0.9 -0.1 -1.3
supernatant control Vogels_fungal_media 0.1X -1.3 -0.5 +0.5 +0.1 -0.2
phage 24hr PHYTO4 MOI 10 +0.3 +0.4 -0.4 +0.0 -1.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.1 +0.5 -0.5 +0.2 -0.2
phage 36hr PHYTO11 MOI 0.1 +1.2 -0.3 +0.1 -1.0 -1.2
phage 36hr PHYTO6 MOI 10 +0.8 -0.8 +0.3 -0.5 -0.7
carbon source D-Galacturonic Acid (C) +0.9 -0.1 -0.6 -0.6 -0.5
phage 24hr PHYTO2 MOI 0.1 +0.7 +0.0 +0.6 -0.4 -1.3
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.8 +0.0 -0.6 +1.3 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +1.3 +0.1 -0.2 -0.8 -0.3
nitrogen source L-Alanine (N) +0.6 -0.4 +0.7 -0.5 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.4 -0.7 +0.4 +0.9 -0.5
lb_dap plate control LB_DAP agar plate, outgrowth in LB -0.6 +0.4 +1.1 -0.1 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.5 +0.6 +0.9 -0.4 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 +0.8 +0.8 -0.7 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +1.8 +0.1 +0.3 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +2.2 +0.1 -0.2 -0.4 -0.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +2.2 -0.1 +0.1 -0.2 -0.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +2.8 +0.2 -0.1 -0.3 -0.1
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +1.2 +0.5 +0.2 +0.6 -0.1
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