Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS23730 and BPHYT_RS23735 are separated by 133 nucleotidesBPHYT_RS23735 and BPHYT_RS23740 are separated by 185 nucleotidesBPHYT_RS23740 and BPHYT_RS23745 are separated by 277 nucleotidesBPHYT_RS23745 and BPHYT_RS23750 are separated by 202 nucleotides BPHYT_RS23730: BPHYT_RS23730 - multidrug DMT transporter permease, at 891,286 to 892,158 _RS23730 BPHYT_RS23735: BPHYT_RS23735 - ABC transporter, at 892,292 to 893,851 _RS23735 BPHYT_RS23740: BPHYT_RS23740 - AraC family transcriptional regulator, at 894,037 to 894,792 _RS23740 BPHYT_RS23745: BPHYT_RS23745 - hypothetical protein, at 895,070 to 895,306 _RS23745 BPHYT_RS23750: BPHYT_RS23750 - phosphonate ABC transporter substrate-binding protein, at 895,509 to 896,597 _RS23750
Group Condition BPHYT_RS23730 BPHYT_RS23735 BPHYT_RS23740 BPHYT_RS23745 BPHYT_RS23750
phage 36hr PHYTO2 MOI 1 -0.9 -0.5 -1.7 N.D. -1.5
stress Bacitracin 0.25 mg/ml +0.2 +0.2 -3.9 N.D. -0.0
phage 36hr PHYTO10 MOI 1 -1.8 -0.5 -1.0 N.D. -0.2
phage PHYTO3 MOI 0.1 -0.8 -0.0 -2.1 N.D. -0.3
phage 24hr PHYTO3 MOI 10 -0.1 +0.5 -3.0 N.D. -0.2
phage P. phyto PHYTO2 MOI 1 -1.1 +0.0 -1.9 N.D. +0.2
phage P. phyto corn MOI 0.1 -1.2 -0.6 -0.7 N.D. -0.1
carbon source Phenylacetic 5 mM (C) -0.1 -0.6 -1.4 N.D. -0.5
phage 36hr PHYTO8 MOI 10 -0.6 +0.1 -2.1 N.D. +0.0
pH LB, pH4 -0.0 -0.0 -2.5 N.D. -0.0
phage 36hr PHYTO3 MOI 1 -0.0 -0.6 -1.6 N.D. -0.2
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -1.1 +0.1 -0.9 N.D. -0.4
phage P. phyto PHYTO2 MOI 1 -1.3 -0.3 -0.8 N.D. +0.1
phage 36hr PHYTO2 MOI 1 +0.3 -0.0 -2.4 N.D. -0.1
stress Lomefloxacin 0.001 mM +0.3 +0.2 -2.7 N.D. -0.1
phage 24hr PHYTO7 MOI 1 -1.3 -0.4 -0.8 N.D. +0.3
phage 36hr PHYTO3 MOI 1 -0.6 -0.1 -1.3 N.D. -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -0.1 -0.1 -1.2 N.D. -0.6
phage 36hr PHYTO8 MOI 0.1 -1.2 +0.0 -0.5 N.D. -0.3
phage 24hr PHYTO11 MOI 0.1 -0.1 -0.1 -1.3 N.D. -0.5
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -1.2 +0.2 -0.5 N.D. -0.1
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.5 +0.3 -1.1 N.D. -0.3
phage P. phyto PHYTO2 MOI 0.1 +0.4 -0.5 -0.9 N.D. -0.5
pH LB, pH4 +0.4 -0.1 -1.7 N.D. +0.1
phage 36hr PHYTO2 MOI 0.1 -1.0 +0.3 -0.5 N.D. +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.7 -0.2 -0.3 N.D. -0.9
phage 24hr PHYTO3 MOI 10 +0.6 +0.3 -0.8 N.D. -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.7 +0.5 -0.4 N.D. +0.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +0.2 +1.1 N.D. -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.9 -0.1 +0.3 N.D. -0.5
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