Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS23710 and BPHYT_RS23715 are separated by 443 nucleotidesBPHYT_RS23715 and BPHYT_RS23720 are separated by 91 nucleotidesBPHYT_RS23720 and BPHYT_RS23725 are separated by 329 nucleotidesBPHYT_RS23725 and BPHYT_RS23730 are separated by 261 nucleotides BPHYT_RS23710: BPHYT_RS23710 - hypothetical protein, at 887,696 to 888,046 _RS23710 BPHYT_RS23715: BPHYT_RS23715 - hypothetical protein, at 888,490 to 888,756 _RS23715 BPHYT_RS23720: BPHYT_RS23720 - nH(3)-dependent NAD(+) synthetase, at 888,848 to 889,708 _RS23720 BPHYT_RS23725: BPHYT_RS23725 - hypothetical protein, at 890,038 to 891,024 _RS23725 BPHYT_RS23730: BPHYT_RS23730 - multidrug DMT transporter permease, at 891,286 to 892,158 _RS23730
Group Condition BPHYT_RS23710 BPHYT_RS23715 BPHYT_RS23720 BPHYT_RS23725 BPHYT_RS23730
phage 36hr PHYTO10 MOI 1 N.D. N.D. +0.0 -0.2 -1.8
phage 24hr PHYTO6 MOI 0.1 N.D. N.D. -0.0 -0.3 -1.6
phage P. phyto PHYTO2 MOI 1 N.D. N.D. -0.4 -0.1 -1.3
phage P. phyto corn MOI 0.1 N.D. N.D. -0.2 -0.3 -1.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. N.D. +0.0 -0.9 -0.8
phage 24hr PHYTO6 MOI 0.1 N.D. N.D. -0.2 -0.9 -0.5
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. N.D. -0.1 -0.2 -1.2
phage 24hr PHYTO11 MOI 0.1 N.D. N.D. -0.3 -1.2 -0.1
nophagecontrol 24hr Phyto Lib only N.D. N.D. -0.2 -0.7 -0.6
phage P. phyto PHYTO2 MOI 0.1 N.D. N.D. -0.2 -1.7 +0.4
phage 36hr PHYTO1 MOI 1 N.D. N.D. -0.0 -0.4 -1.0
phage PHYTO3 MOI 0.1 N.D. N.D. -0.4 -0.1 -0.8
nophagecontrol 36hr Phyto Lib only N.D. N.D. +0.0 -0.4 -1.0
motility outer cut, LB soft agar motility assay N.D. N.D. +0.1 -0.9 -0.5
motility inner cut, LB soft agar motility assay N.D. N.D. +0.2 -0.7 -0.7
phage 36hr PHYTO6 MOI 10 N.D. N.D. +0.2 -0.2 -1.0
phage 24hr PHYTO7 MOI 1 N.D. N.D. +0.0 +0.2 -1.3
phage 36hr PHYTO8 MOI 0.1 N.D. N.D. +0.3 -0.1 -1.2
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. N.D. +0.3 -0.3 -0.8
phage 36hr PHYTO2 MOI 1 N.D. N.D. -0.1 +0.5 -0.9
nitrogen source D-Alanine (N) N.D. N.D. +0.6 -1.0 +0.0
phage 36hr PHYTO9 MOI 1 N.D. N.D. +0.1 +0.5 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -0.2 +1.4 -0.7
phage 36hr PHYTO3 MOI 1 N.D. N.D. +0.4 +0.7 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.5 -0.4 +0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.1 -0.1 +1.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.3 -0.3 +1.0
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X N.D. N.D. +0.4 +0.7 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. +0.4 +0.6 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. +0.3 +0.2 +1.0
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