Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS23490 and BPHYT_RS23495 are separated by 214 nucleotidesBPHYT_RS23495 and BPHYT_RS23500 are separated by 134 nucleotidesBPHYT_RS23500 and BPHYT_RS23505 are separated by 214 nucleotidesBPHYT_RS23505 and BPHYT_RS23510 are separated by 164 nucleotides BPHYT_RS23490: BPHYT_RS23490 - hypothetical protein, at 836,450 to 836,752 _RS23490 BPHYT_RS23495: BPHYT_RS23495 - thioredoxin reductase, at 836,967 to 838,700 _RS23495 BPHYT_RS23500: BPHYT_RS23500 - hypothetical protein, at 838,835 to 840,013 _RS23500 BPHYT_RS23505: BPHYT_RS23505 - hypothetical protein, at 840,228 to 840,611 _RS23505 BPHYT_RS23510: BPHYT_RS23510 - AMP-dependent synthetase, at 840,776 to 842,320 _RS23510
Group Condition BPHYT_RS23490 BPHYT_RS23495 BPHYT_RS23500 BPHYT_RS23505 BPHYT_RS23510
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.6 -0.2 N.D. -0.9
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. +0.5 -1.6 N.D. -0.5
carbon source Phenylacetic 5 mM (C) N.D. +0.2 -1.1 N.D. -0.6
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. -0.6 -0.8 N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.4 -1.1 N.D. -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -0.9 -0.6 N.D. +0.5
phage 36hr PHYTO11 MOI 0.1 N.D. +0.3 -1.1 N.D. +0.3
phage P. phyto PHYTO2 MOI 0.1 N.D. -0.3 -0.8 N.D. +0.7
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. -0.9 +0.9 N.D. -0.3
phage 24hr PHYTO3 MOI 10 N.D. -0.8 -0.1 N.D. +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.1 +0.7 N.D. -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.8 +0.7 N.D. +0.2
phage 36hr PHYTO7 MOI 0.1 N.D. -0.8 +0.3 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.1 -0.7 N.D. +0.8
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. -0.7 +0.8 N.D. +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.4 -0.2 N.D. +1.1
phage 24hr PHYTO6 MOI 10 N.D. +0.4 +0.7 N.D. -0.6
nitrogen source L-Alanine (N) N.D. +1.0 -0.6 N.D. +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +1.6 -0.6 N.D. -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.9 -0.6 N.D. +0.6
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.4 +0.4 N.D. +1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +1.0 -0.5 N.D. +0.5
phage 36hr PHYTO3 MOI 1 N.D. +0.3 +0.6 N.D. +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +1.5 +0.2 N.D. +0.0
carbon source Protocatechuic Acid (C) N.D. +0.8 +0.9 N.D. +0.1
phage 36hr PHYTO11 MOI 0.1 N.D. +0.5 +0.9 N.D. +0.5
carbon source adipate (C) N.D. +1.1 +0.6 N.D. +0.3
carbon source Dodecandioic (C) N.D. +0.6 +1.1 N.D. +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.5 +0.7 N.D. +0.8
carbon source Suberic (C) N.D. +0.9 +1.1 N.D. +0.4
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BPHYT_RS23490
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BPHYT_RS23495
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BPHYT_RS23510
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