Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS23485 and BPHYT_RS23490 are separated by 105 nucleotidesBPHYT_RS23490 and BPHYT_RS23495 are separated by 214 nucleotidesBPHYT_RS23495 and BPHYT_RS23500 are separated by 134 nucleotidesBPHYT_RS23500 and BPHYT_RS23505 are separated by 214 nucleotides BPHYT_RS23485: BPHYT_RS23485 - glyoxalase, at 835,922 to 836,344 _RS23485 BPHYT_RS23490: BPHYT_RS23490 - hypothetical protein, at 836,450 to 836,752 _RS23490 BPHYT_RS23495: BPHYT_RS23495 - thioredoxin reductase, at 836,967 to 838,700 _RS23495 BPHYT_RS23500: BPHYT_RS23500 - hypothetical protein, at 838,835 to 840,013 _RS23500 BPHYT_RS23505: BPHYT_RS23505 - hypothetical protein, at 840,228 to 840,611 _RS23505
Group Condition BPHYT_RS23485 BPHYT_RS23490 BPHYT_RS23495 BPHYT_RS23500 BPHYT_RS23505
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. -0.9 -0.6 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.8 -0.8 N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. N.D. -0.6 -0.8 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. +0.5 -1.6 N.D.
carbon source Phenylacetic 5 mM (C) N.D. N.D. +0.2 -1.1 N.D.
phage 36hr PHYTO11 MOI 0.1 N.D. N.D. +0.3 -1.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.4 -1.1 N.D.
carbon source Malonic (C) N.D. N.D. +0.4 -0.8 N.D.
phage 36hr PHYTO10 MOI 10 N.D. N.D. -0.9 +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -0.8 +0.7 N.D.
phage P. phyto PHYTO2 MOI 1 N.D. N.D. -0.7 +0.6 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. N.D. +0.6 -0.6 N.D.
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.9 +0.9 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. N.D. -0.7 +0.8 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.9 -0.6 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. N.D. +0.8 -0.5 N.D.
nitrogen source L-Alanine (N) N.D. N.D. +1.0 -0.6 N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X N.D. N.D. +0.9 -0.5 N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. N.D. +0.9 -0.4 N.D.
motility outer cut, LB soft agar motility assay N.D. N.D. -0.5 +1.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +1.0 -0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. +1.6 -0.6 N.D.
nitrogen source L-Glutamic (N) N.D. N.D. +0.7 +0.7 N.D.
carbon source D-Glucose (C) N.D. N.D. +0.6 +0.8 N.D.
phage 36hr PHYTO11 MOI 0.1 N.D. N.D. +0.5 +0.9 N.D.
carbon source Dodecandioic (C) N.D. N.D. +0.6 +1.1 N.D.
carbon source adipate (C) N.D. N.D. +1.1 +0.6 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. +1.5 +0.2 N.D.
carbon source Protocatechuic Acid (C) N.D. N.D. +0.8 +0.9 N.D.
carbon source Suberic (C) N.D. N.D. +0.9 +1.1 N.D.
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