Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS23445 and BPHYT_RS23450 are separated by 216 nucleotidesBPHYT_RS23450 and BPHYT_RS23455 are separated by 131 nucleotidesBPHYT_RS23455 and BPHYT_RS23460 are separated by 46 nucleotidesBPHYT_RS23460 and BPHYT_RS23465 are separated by 213 nucleotides BPHYT_RS23445: BPHYT_RS23445 - hexuronate transporter, at 827,844 to 829,136 _RS23445 BPHYT_RS23450: BPHYT_RS23450 - GntR family transcriptional regulator, at 829,353 to 830,066 _RS23450 BPHYT_RS23455: BPHYT_RS23455 - phosphorylase, at 830,198 to 831,106 _RS23455 BPHYT_RS23460: BPHYT_RS23460 - AraC family transcriptional regulator, at 831,153 to 832,109 _RS23460 BPHYT_RS23465: BPHYT_RS23465 - aliphatic nitrilase, at 832,323 to 833,309 _RS23465
Group Condition BPHYT_RS23445 BPHYT_RS23450 BPHYT_RS23455 BPHYT_RS23460 BPHYT_RS23465
phage 24hr PHYTO9 MOI 0.1 -1.7 N.D. -0.4 -3.6 -0.4
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.4 N.D. -0.8 -3.0 -0.7
phage 36hr PHYTO4 MOI 1 -3.3 N.D. -0.5 -0.8 -0.1
phage 36hr PHYTO8 MOI 10 -1.9 N.D. +0.5 -2.5 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 N.D. -0.4 -2.1 -0.8
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.9 N.D. -2.4 -0.7 +0.2
phage 24hr PHYTO11 MOI 0.1 -3.3 N.D. -0.2 +0.5 -0.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -2.5 N.D. +0.2 -0.9 +0.5
tn7 efficiency Outgrowth after Tn7 transformation -0.5 N.D. +0.9 -2.6 -0.5
phage 36hr PHYTO1 MOI 1 -3.9 N.D. +0.8 -0.1 +0.6
phage 24hr PHYTO4 MOI 0.1 -3.9 N.D. +0.4 +0.9 +0.1
phage 36hr PHYTO3 MOI 0.1 -0.4 N.D. +1.0 -2.7 -0.1
phage 36hr PHYTO11 MOI 1 -0.5 N.D. +0.9 -2.7 +0.3
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +1.2 N.D. +0.5 -3.7 +0.1
phage 36hr PHYTO2 MOI 1 -0.4 N.D. +1.1 -2.6 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.8 N.D. -4.3 +1.6 -0.0
phage 24hr PHYTO3 MOI 10 +0.7 N.D. +1.1 -3.0 -0.4
phage 24hr PHYTO3 MOI 0.1 -0.1 N.D. +1.5 -2.6 +0.2
phage 36hr PHYTO10 MOI 1 -2.4 N.D. +1.2 +0.7 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -2.6 N.D. +0.6 +0.6 +1.5
phage P. phyto PHYTO2 MOI 1 +1.2 N.D. -1.9 +0.8 +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.7 N.D. +1.7 -1.3 +0.4
phage 24hr PHYTO MOI 0.1 -1.2 N.D. +1.3 +0.8 +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.0 N.D. -1.4 +1.4 +0.5
lb_nosalt LB_noSalt +1.2 N.D. -1.3 +1.8 +0.0
phage 36hr PHYTO1 MOI 10 -1.4 N.D. +1.7 +1.1 +0.4
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +1.3 N.D. -0.8 +2.0 -0.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.7 N.D. -0.3 +0.9 +1.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.5 N.D. +2.0 +1.6 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.5 N.D. -0.6 +1.9 +1.2
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