Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS23385 and BPHYT_RS23390 are separated by 518 nucleotidesBPHYT_RS23390 and BPHYT_RS23395 are separated by 312 nucleotidesBPHYT_RS23395 and BPHYT_RS23400 are separated by 183 nucleotidesBPHYT_RS23400 and BPHYT_RS23405 are separated by 177 nucleotides BPHYT_RS23385: BPHYT_RS23385 - group 1 glycosyl transferase, at 807,719 to 808,894 _RS23385 BPHYT_RS23390: BPHYT_RS23390 - hypothetical protein, at 809,413 to 810,423 _RS23390 BPHYT_RS23395: BPHYT_RS23395 - glycosyl transferase, at 810,736 to 811,872 _RS23395 BPHYT_RS23400: BPHYT_RS23400 - GntR family transcriptional regulator, at 812,056 to 812,754 _RS23400 BPHYT_RS23405: BPHYT_RS23405 - glucosamine--fructose-6-phosphate aminotransferase, at 812,932 to 814,749 _RS23405
Group Condition BPHYT_RS23385 BPHYT_RS23390 BPHYT_RS23395 BPHYT_RS23400 BPHYT_RS23405
phage 36hr PHYTO2 MOI 1 -2.9 +0.0 -1.9 N.D. N.D.
phage 24hr PHYTO3 MOI 0.1 -2.0 +0.2 -1.0 N.D. N.D.
phage 36hr PHYTO10 MOI 10 -0.9 -0.0 -1.7 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.7 +0.4 -2.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.1 -0.0 -1.2 N.D. N.D.
phage 36hr PHYTO11 MOI 1 -2.8 +0.3 +0.2 N.D. N.D.
phage 36hr PHYTO MOI 0.1 -0.3 +0.2 -2.0 N.D. N.D.
phage 24hr PHYTO6 MOI 0.1 -2.8 +0.2 +0.7 N.D. N.D.
carbon source D-Glucose (C) -0.3 +0.4 -2.0 N.D. N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.2 +0.2 -1.8 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 +0.5 -1.1 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.9 -0.7 -1.3 N.D. N.D.
nophagecontrol 36hr Phyto Lib only -1.9 +0.2 +0.7 N.D. N.D.
phage 36hr PHYTO3 MOI 0.1 -0.2 +0.8 -1.5 N.D. N.D.
phage 36hr PHYTO8 MOI 10 +0.6 +0.4 -1.7 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.9 -0.2 -1.2 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 +0.4 -1.6 N.D. N.D.
phage 24hr PHYTO6 MOI 10 -0.7 -0.8 +1.1 N.D. N.D.
phage P. phyto PHYTO2 MOI 0.1 -1.2 -0.3 +1.1 N.D. N.D.
phage 24hr PHYTO3 MOI 10 -2.4 +1.0 +1.5 N.D. N.D.
phage 24hr PHYTO10 MOI 1 -1.3 +0.1 +1.4 N.D. N.D.
nitrogen source L-Lysine (N) +0.5 -1.1 +0.8 N.D. N.D.
phage 24hr PHYTO4 MOI 10 -0.9 +1.6 -0.3 N.D. N.D.
phage 36hr PHYTO4 MOI 1 -1.2 +0.4 +1.5 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.4 -0.2 +1.8 N.D. N.D.
supernatant control Vogels_fungal_media; pH 5.5 +1.3 -1.0 +1.0 N.D. N.D.
carbon source L-Arabinose (C) +1.1 -0.3 +0.8 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.1 +0.6 +1.7 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.8 +0.0 +1.4 N.D. N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.5 +0.2 +1.6 N.D. N.D.
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