Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS23285 and BPHYT_RS23290 are separated by 263 nucleotidesBPHYT_RS23290 and BPHYT_RS23295 are separated by 3 nucleotidesBPHYT_RS23295 and BPHYT_RS23300 are separated by 52 nucleotidesBPHYT_RS23300 and BPHYT_RS23305 are separated by 45 nucleotides BPHYT_RS23285: BPHYT_RS23285 - hypothetical protein, at 789,000 to 789,278 _RS23285 BPHYT_RS23290: BPHYT_RS23290 - hypothetical protein, at 789,542 to 789,730 _RS23290 BPHYT_RS23295: BPHYT_RS23295 - O-methyltransferase, at 789,734 to 790,348 _RS23295 BPHYT_RS23300: BPHYT_RS23300 - IclR family transcriptional regulator, at 790,401 to 791,012 _RS23300 BPHYT_RS23305: BPHYT_RS23305 - hypothetical protein, at 791,058 to 791,600 _RS23305
Group Condition BPHYT_RS23285 BPHYT_RS23290 BPHYT_RS23295 BPHYT_RS23300 BPHYT_RS23305
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. N.D. -0.5 N.D. -0.6
phage 36hr PHYTO1 MOI 1 N.D. N.D. -0.1 N.D. -0.9
phage 36hr PHYTO1 MOI 10 N.D. N.D. -0.5 N.D. -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -0.6 N.D. -0.2
phage 24hr PHYTO1 MOI 10 N.D. N.D. -0.3 N.D. -0.4
phage 36hr PHYTO2 MOI 0.1 N.D. N.D. -0.0 N.D. -0.7
phage 24hr PHYTO4 MOI 10 N.D. N.D. +0.1 N.D. -0.7
phage 24hr PHYTO2 MOI 0.1 N.D. N.D. -0.2 N.D. -0.5
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. N.D. -0.0 N.D. -0.6
phage 24hr PHYTO6 MOI 10 N.D. N.D. -0.6 N.D. +0.2
phage 36hr PHYTO8 MOI 10 N.D. N.D. +0.2 N.D. -0.5
phage 24hr PHYTO11 MOI 1 N.D. N.D. +0.3 N.D. -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.2 N.D. -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.4 N.D. +0.2
nitrogen source L-Lysine (N) N.D. N.D. +0.4 N.D. -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.5 N.D. +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. -0.3 N.D. +0.5
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. N.D. +0.5 N.D. -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. +0.9 N.D. -0.4
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. N.D. +0.5 N.D. +0.2
tn7 efficiency Outgrowth after Tn7 transformation N.D. N.D. +0.4 N.D. +0.3
tn7 efficiency Outgrowth after Tn7 transformation N.D. N.D. +0.4 N.D. +0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.5 N.D. +0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.3 N.D. +0.4
carbon source Malonic (C) N.D. N.D. +0.3 N.D. +0.6
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.8 N.D. +0.0
carbon source Sebacic (C) N.D. N.D. +0.6 N.D. +0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.5 N.D. +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.1 N.D. +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. +0.0 N.D. +1.0
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