Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS23000 and BPHYT_RS23005 are separated by 765 nucleotidesBPHYT_RS23005 and BPHYT_RS23010 are separated by 113 nucleotidesBPHYT_RS23010 and BPHYT_RS23015 are separated by 59 nucleotidesBPHYT_RS23015 and BPHYT_RS23020 are separated by 193 nucleotides BPHYT_RS23000: BPHYT_RS23000 - molybdopterin-dependent oxidoreductase, at 720,276 to 720,872 _RS23000 BPHYT_RS23005: BPHYT_RS23005 - carboxymuconolactone decarboxylase, at 721,638 to 722,186 _RS23005 BPHYT_RS23010: BPHYT_RS23010 - transcriptional regulator, at 722,300 to 723,157 _RS23010 BPHYT_RS23015: BPHYT_RS23015 - porin, at 723,217 to 724,410 _RS23015 BPHYT_RS23020: BPHYT_RS23020 - alcohol dehydrogenase, at 724,604 to 725,959 _RS23020
Group Condition BPHYT_RS23000 BPHYT_RS23005 BPHYT_RS23010 BPHYT_RS23015 BPHYT_RS23020
phage 36hr PHYTO2 MOI 1 N.D. N.D. +0.1 -0.9 -1.0
nophagecontrol 24hr Phyto Lib only N.D. N.D. -0.4 -0.9 -0.5
phage 36hr PHYTO3 MOI 1 N.D. N.D. -0.6 -0.7 -0.4
phage 24hr PHYTO3 MOI 10 N.D. N.D. -0.6 +0.1 -1.1
phage 36hr PHYTO8 MOI 10 N.D. N.D. -0.1 -0.9 -0.4
phage 36hr PHYTO9 MOI 1 N.D. N.D. -0.1 -0.1 -1.0
phage 24hr PHYTO3 MOI 1 N.D. N.D. -0.4 -0.7 -0.0
phage 24hr PHYTO1 MOI 1 N.D. N.D. -0.0 -0.4 -0.8
phage 24hr PHYTO11 MOI 1 N.D. N.D. -0.5 +0.1 -0.8
phage 24hr PHYTO8 MOI 1 N.D. N.D. -0.4 +0.2 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -0.8 -0.6 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.6 -0.8 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -1.1 -0.7 +1.1
nitrogen source L-Phenylalanine (N) N.D. N.D. -0.0 +0.5 -0.8
carbon source Phenylacetic 5 mM (C) N.D. N.D. -0.5 +0.5 -0.4
phage 24hr PHYTO4 MOI 10 N.D. N.D. -0.3 +0.7 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. N.D. +0.6 -0.3 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. N.D. +0.9 -0.1 -0.7
carbon source D-Glucose (C) N.D. N.D. +0.5 +0.3 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.6 +0.4 +0.6
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) N.D. N.D. +0.2 +0.7 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. N.D. +0.5 +0.6 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. +0.8 +0.3 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. +0.6 +0.6 -0.4
supernatant control Vogels_fungal_media; pH 5.5 N.D. N.D. -0.1 +1.3 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.8 +0.5 +1.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.1 +0.5 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. +0.6 +0.4 +0.2
supernatant control Vogels_fungal_media 0.1X N.D. N.D. +0.0 +0.6 +0.6
carbon source Malonic (C) N.D. N.D. +0.1 +1.3 +0.3
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BPHYT_RS23000
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