Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS22965 and BPHYT_RS22970 are separated by 82 nucleotidesBPHYT_RS22970 and BPHYT_RS22975 are separated by 273 nucleotidesBPHYT_RS22975 and BPHYT_RS22980 are separated by 52 nucleotidesBPHYT_RS22980 and BPHYT_RS22985 are separated by 103 nucleotides BPHYT_RS22965: BPHYT_RS22965 - ligand-gated channel protein, at 710,220 to 712,634 _RS22965 BPHYT_RS22970: BPHYT_RS22970 - hypothetical protein, at 712,717 to 713,127 _RS22970 BPHYT_RS22975: BPHYT_RS22975 - hypothetical protein, at 713,401 to 715,545 _RS22975 BPHYT_RS22980: BPHYT_RS22980 - LysR family transcriptional regulator, at 715,598 to 716,494 _RS22980 BPHYT_RS22985: BPHYT_RS22985 - hypothetical protein, at 716,598 to 717,713 _RS22985
Group Condition BPHYT_RS22965 BPHYT_RS22970 BPHYT_RS22975 BPHYT_RS22980 BPHYT_RS22985
phage 24hr PHYTO3 MOI 10 -0.1 N.D. -0.3 N.D. -0.8
nitrogen source L-Glutamic (N) -0.0 N.D. -0.4 N.D. -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 N.D. -0.3 N.D. -0.5
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.7 N.D. -0.2 N.D. -0.1
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.1 N.D. -0.7 N.D. -0.4
phage 36hr PHYTO2 MOI 1 +0.1 N.D. -0.0 N.D. -0.9
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.1 N.D. -0.2 N.D. -0.7
phage 24hr PHYTO9 MOI 10 -0.1 N.D. +0.2 N.D. -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 N.D. -0.4 N.D. -0.3
phage P. phyto corn MOI 1 -0.1 N.D. +0.3 N.D. -0.5
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.4 N.D. -0.2 N.D. -0.5
phage 36hr PHYTO MOI 0.1 +0.1 N.D. +0.3 N.D. -0.6
phage 36hr PHYTO3 MOI 0.1 +0.2 N.D. +0.2 N.D. -0.5
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.5 N.D. -0.2 N.D. -0.3
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.5 N.D. +0.1 N.D. -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 N.D. +0.4 N.D. -0.4
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.5 N.D. +0.1 N.D. -0.4
phage 36hr PHYTO6 MOI 0.1 +0.4 N.D. +0.2 N.D. -0.4
carbon source propionate (C) +0.2 N.D. -0.4 N.D. +0.5
phage 36hr PHYTO1 MOI 1 +0.5 N.D. +0.1 N.D. -0.4
carbon source D-Glucose (C) +0.3 N.D. -0.3 N.D. +0.4
phage PHYTO3 MOI 0.1 +0.4 N.D. -0.3 N.D. +0.2
phage 24hr PHYTO7 MOI 1 -0.4 N.D. +0.5 N.D. +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 N.D. +0.4 N.D. -0.5
r2a control R2A control -0.2 N.D. +0.1 N.D. +0.6
phage 36hr PHYTO9 MOI 1 -0.1 N.D. +0.5 N.D. +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.8 N.D. -0.1 N.D. +0.3
stress Benzethonium chloride 0.0016 mM +1.0 N.D. -0.1 N.D. +0.1
stress Cephalothin 2 mg/ml +1.1 N.D. +0.0 N.D. +0.0
phage 24hr PHYTO6 MOI 10 -0.0 N.D. +0.3 N.D. +0.9
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