Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS22950 and BPHYT_RS22955 are separated by 7 nucleotidesBPHYT_RS22955 and BPHYT_RS22960 are separated by 63 nucleotidesBPHYT_RS22960 and BPHYT_RS22965 are separated by 74 nucleotidesBPHYT_RS22965 and BPHYT_RS22970 are separated by 82 nucleotides BPHYT_RS22950: BPHYT_RS22950 - von Willebrand factor A, at 706,467 to 707,822 _RS22950 BPHYT_RS22955: BPHYT_RS22955 - isoprenylcysteine carboxyl methyltransferase, at 707,830 to 708,543 _RS22955 BPHYT_RS22960: BPHYT_RS22960 - peptidase, at 708,607 to 710,145 _RS22960 BPHYT_RS22965: BPHYT_RS22965 - ligand-gated channel protein, at 710,220 to 712,634 _RS22965 BPHYT_RS22970: BPHYT_RS22970 - hypothetical protein, at 712,717 to 713,127 _RS22970
Group Condition BPHYT_RS22950 BPHYT_RS22955 BPHYT_RS22960 BPHYT_RS22965 BPHYT_RS22970
phage 36hr PHYTO8 MOI 10 -0.3 -3.0 N.D. -0.4 N.D.
phage 36hr PHYTO11 MOI 1 -0.4 -2.3 N.D. +0.1 N.D.
nophagecontrol 36hr Phyto Lib Only -0.4 -2.0 N.D. +0.3 N.D.
phage 24hr PHYTO2 MOI 1 +0.0 -1.2 N.D. -0.4 N.D.
phage 24hr PHYTO10 MOI 10 -0.5 -1.3 N.D. +0.2 N.D.
phage 24hr PHYTO3 MOI 0.1 -0.6 -1.1 N.D. +0.2 N.D.
phage 36hr PHYTO11 MOI 1 -0.1 -1.2 N.D. -0.1 N.D.
phage 36hr PHYTO2 MOI 1 -0.4 -1.0 N.D. -0.0 N.D.
phage P. phyto PHYTO2 MOI 1 -0.3 -1.2 N.D. +0.2 N.D.
phage 24hr PHYTO10 MOI 1 -0.2 -1.3 N.D. +0.2 N.D.
phage 24hr PHYTO1 MOI 10 -1.0 -0.2 N.D. -0.0 N.D.
phage 36hr PHYTO MOI 0.1 -0.1 -1.1 N.D. +0.1 N.D.
phage 36hr PHYTO6 MOI 0.1 +0.0 -1.4 N.D. +0.3 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.5 -0.5 N.D. +0.2 N.D.
phage 24hr PHYTO6 MOI 0.1 -0.3 -0.7 N.D. +0.2 N.D.
phage 36hr PHYTO4 MOI 0.1 -0.2 -1.0 N.D. +0.5 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.6 -0.5 N.D. +0.4 N.D.
phage 36hr PHYTO6 MOI 10 -0.5 -0.5 N.D. +0.4 N.D.
phage P. phyto PHYTO2 MOI 0.1 +0.1 -1.1 N.D. +0.4 N.D.
phage 36hr PHYTO1 MOI 1 -0.7 -0.2 N.D. +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 -0.9 N.D. +0.3 N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.5 -0.3 N.D. +0.5 N.D.
carbon source 2-Deoxy-D-Ribose (C) +0.4 -0.8 N.D. +0.1 N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.0 +0.7 N.D. -0.7 N.D.
carbon source adipate (C) +0.5 -0.6 N.D. +0.1 N.D.
carbon source Sebacic (C) +0.7 -0.5 N.D. +0.1 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.4 +0.6 N.D. +0.3 N.D.
stress Benzethonium chloride 0.0016 mM -0.2 -0.0 N.D. +1.0 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.3 +0.9 N.D. -0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.7 +0.7 N.D. +0.5 N.D.
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