Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS22895 and BPHYT_RS22900 are separated by 37 nucleotidesBPHYT_RS22900 and BPHYT_RS22905 are separated by 88 nucleotidesBPHYT_RS22905 and BPHYT_RS22910 are separated by 24 nucleotidesBPHYT_RS22910 and BPHYT_RS22915 are separated by 46 nucleotides BPHYT_RS22895: BPHYT_RS22895 - peptidase A24, at 696,070 to 696,606 _RS22895 BPHYT_RS22900: BPHYT_RS22900 - pilus assembly protein CpaB, at 696,644 to 697,477 _RS22900 BPHYT_RS22905: BPHYT_RS22905 - secretin, at 697,566 to 699,353 _RS22905 BPHYT_RS22910: BPHYT_RS22910 - hypothetical protein, at 699,378 to 699,671 _RS22910 BPHYT_RS22915: BPHYT_RS22915 - pilus assembly protein CpaE, at 699,718 to 700,920 _RS22915
Group Condition BPHYT_RS22895 BPHYT_RS22900 BPHYT_RS22905 BPHYT_RS22910 BPHYT_RS22915
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. N.D. +0.3 -4.8 -0.5
carbon source adipate (C) N.D. N.D. +0.0 -3.5 -0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +0.2 -3.4 -0.3
phage 24hr PHYTO2 MOI 1 N.D. N.D. -0.3 -2.1 -1.0
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. N.D. +0.1 -3.4 -0.1
phage PHYTO3 MOI 0.1 N.D. N.D. -0.2 -2.5 -0.6
nophagecontrol 24hr Phyto Lib Only N.D. N.D. +0.4 -2.0 -1.6
phage 24hr PHYTO3 MOI 10 N.D. N.D. -0.0 -2.2 -0.9
phage 24hr PHYTO2 MOI 0.1 N.D. N.D. +0.2 -2.0 -1.2
phage 24hr PHYTO3 MOI 10 N.D. N.D. +0.1 -2.3 -0.8
nitrogen source L-Lysine (N) N.D. N.D. -0.1 -2.2 -0.6
phage 24hr PHYTO3 MOI 0.1 N.D. N.D. -0.0 -2.0 -0.9
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. N.D. +0.3 -3.0 -0.1
phage 24hr PHYTO6 MOI 0.1 N.D. N.D. +0.2 -2.0 -0.9
phage 24hr PHYTO6 MOI 10 N.D. N.D. -0.3 -1.8 -0.6
phage 24hr PHYTO10 MOI 1 N.D. N.D. +0.1 -2.0 -0.7
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. N.D. -0.6 -2.1 +0.1
phage 24hr PHYTO3 MOI 1 N.D. N.D. +0.2 -2.0 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.2 -1.9 -0.4
phage 24hr PHYTO6 MOI 0.1 N.D. N.D. +0.3 -2.0 -0.5
phage 36hr PHYTO4 MOI 1 N.D. N.D. +0.5 -1.7 -0.9
phage 24hr PHYTO2 MOI 0.1 N.D. N.D. +0.3 -1.9 -0.5
phage 36hr PHYTO4 MOI 0.1 N.D. N.D. +0.4 -1.8 -0.5
nophagecontrol 36hr Phyto Lib Only N.D. N.D. +0.4 -1.8 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. +0.2 -2.2 +0.5
phage 36hr PHYTO3 MOI 1 N.D. N.D. +0.6 -2.0 -0.1
phage 36hr PHYTO11 MOI 1 N.D. N.D. +0.1 -2.0 +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.1 -2.0 +0.7
phage 24hr PHYTO1 MOI 10 N.D. N.D. +0.5 -1.9 +0.4
carbon source Suberic (C) N.D. N.D. +0.7 +1.4 -0.6
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