Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS22745 and BPHYT_RS22750 are separated by 446 nucleotidesBPHYT_RS22750 and BPHYT_RS22755 are separated by 250 nucleotidesBPHYT_RS22755 and BPHYT_RS22760 are separated by 193 nucleotidesBPHYT_RS22760 and BPHYT_RS22765 are separated by 11 nucleotides BPHYT_RS22745: BPHYT_RS22745 - sugar ABC transporter, at 659,938 to 660,942 _RS22745 BPHYT_RS22750: BPHYT_RS22750 - Flagellar brake protein YcgR 2, at 661,389 to 662,162 _RS22750 BPHYT_RS22755: BPHYT_RS22755 - LacI family transcription regulator, at 662,413 to 663,444 _RS22755 BPHYT_RS22760: BPHYT_RS22760 - sugar ABC transporter ATP-binding protein, at 663,638 to 664,792 _RS22760 BPHYT_RS22765: BPHYT_RS22765 - beta-galactosidase, at 664,804 to 666,786 _RS22765
Group Condition BPHYT_RS22745 BPHYT_RS22750 BPHYT_RS22755 BPHYT_RS22760 BPHYT_RS22765
phage 24hr PHYTO10 MOI 10 N.D. -1.3 -0.1 -1.0 -0.7
phage 24hr PHYTO9 MOI 10 N.D. -1.2 -0.5 -0.1 -1.0
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.0 -0.1 +0.2 -2.9
motility inner cut, LB soft agar motility assay N.D. -0.3 -0.8 -0.1 -1.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. -1.6 -1.2 -0.1 +0.5
phage 24hr PHYTO6 MOI 0.1 N.D. -2.2 +0.4 +0.0 -0.6
nophagecontrol 24hr Phyto Lib Only N.D. -0.8 -0.3 -0.0 -1.1
phage 36hr PHYTO8 MOI 10 N.D. -0.6 +0.4 -0.6 -1.1
phage 36hr PHYTO6 MOI 0.1 N.D. -2.0 +0.4 -0.2 +0.1
phage 24hr PHYTO3 MOI 10 N.D. -0.6 +0.3 +0.2 -1.6
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.7 -0.1 -0.7 -1.5
phage 24hr PHYTO3 MOI 10 N.D. -0.7 +0.0 +0.7 -1.6
phage PHYTO3 MOI 0.1 N.D. -0.5 +0.6 -0.3 -0.9
phage 36hr PHYTO8 MOI 10 N.D. -1.7 +0.5 +0.7 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.3 +0.4 -0.4 -1.3
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. -0.4 -0.8 -0.4 +0.9
phage 24hr PHYTO7 MOI 1 N.D. +0.5 -0.5 +0.4 -1.1
phage 24hr PHYTO1 MOI 10 N.D. -1.5 +0.5 -0.2 +0.6
phage 24hr PHYTO6 MOI 10 N.D. -0.5 -0.1 -0.7 +1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. -1.2 +0.2 +0.7 +0.3
nitrogen source L-Lysine (N) N.D. -0.6 +0.1 -0.5 +1.1
nophagecontrol 36hr Phyto Lib Only N.D. -1.2 +0.0 +0.5 +1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. -0.5 +0.4 -0.4 +0.9
phage 36hr PHYTO4 MOI 1 N.D. -1.3 +1.2 +0.2 +0.3
phage P. phyto corn MOI 1 N.D. -0.5 -0.4 +0.9 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.7 +0.3 -0.9 +0.6
phage 36hr PHYTO9 MOI 1 N.D. -0.7 +0.6 +0.5 +0.5
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. -0.7 +1.4 +0.3 -0.1
carbon source Tween 20 (C) N.D. -0.3 +0.8 +0.4 +0.9
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. -0.2 +7.0 +0.0 +0.2
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