Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS22520 and BPHYT_RS22525 are separated by 375 nucleotidesBPHYT_RS22525 and BPHYT_RS22530 are separated by 43 nucleotidesBPHYT_RS22530 and BPHYT_RS22535 are separated by 18 nucleotidesBPHYT_RS22535 and BPHYT_RS22540 overlap by 8 nucleotides BPHYT_RS22520: BPHYT_RS22520 - RND transporter, at 613,083 to 614,684 _RS22520 BPHYT_RS22525: BPHYT_RS22525 - dioxygenase, at 615,060 to 615,899 _RS22525 BPHYT_RS22530: BPHYT_RS22530 - N5,N10-methylene tetrahydromethanopterin reductase, at 615,943 to 617,349 _RS22530 BPHYT_RS22535: BPHYT_RS22535 - methionine ABC transporter ATP-binding protein, at 617,368 to 618,528 _RS22535 BPHYT_RS22540: BPHYT_RS22540 - metal ABC transporter permease, at 618,521 to 619,177 _RS22540
Group Condition BPHYT_RS22520 BPHYT_RS22525 BPHYT_RS22530 BPHYT_RS22535 BPHYT_RS22540
phage 24hr PHYTO6 MOI 10 -1.1 -0.8 -2.4 N.D. +0.2
phage 36hr PHYTO8 MOI 10 -0.2 -1.5 -1.2 N.D. -0.8
phage 36hr PHYTO2 MOI 1 -0.1 -0.2 -1.5 N.D. -1.7
phage 24hr PHYTO1 MOI 10 +0.2 -0.3 -3.2 N.D. +0.5
phage 36hr PHYTO2 MOI 1 +0.1 -0.3 -2.2 N.D. -0.3
tn7 efficiency Outgrowth after Tn7 transformation -0.4 -0.8 -1.1 N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.0 -0.5 -1.8 N.D. -0.2
phage 36hr PHYTO8 MOI 10 +0.3 -1.5 -1.6 N.D. +0.3
phage 24hr PHYTO4 MOI 0.1 -0.4 -0.3 -2.3 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.3 +0.2 -1.5 N.D. -0.6
phage 36hr PHYTO3 MOI 1 +0.3 -0.1 -1.5 N.D. -0.7
phage 36hr PHYTO8 MOI 0.1 -0.5 +0.3 -0.2 N.D. -1.7
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -0.1 -1.2 N.D. -1.0
motility outer cut, LB soft agar motility assay +0.4 -0.3 -1.4 N.D. -0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -1.0 +0.7 N.D. -1.4
stress Benzethonium chloride 0.0016 mM -2.1 +0.3 +0.3 N.D. -0.2
phage 24hr PHYTO7 MOI 1 -0.5 -0.2 -1.4 N.D. +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X -2.0 +0.0 -0.0 N.D. +0.7
phage 36hr PHYTO6 MOI 1 +0.2 -1.0 -1.1 N.D. +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.2 -1.2 -0.8 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 -0.5 -1.2 N.D. +0.5
motility inner cut, LB soft agar motility assay +0.2 -0.1 -1.5 N.D. +1.7
phage 24hr PHYTO9 MOI 10 -0.8 +0.2 -0.7 N.D. +1.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 +1.2 +1.0 N.D. -1.0
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.8 -0.0 -0.7 N.D. +1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.1 +1.4 +0.5 N.D. -0.7
carbon source Phenylacetic 5 mM (C) +0.4 +0.8 -0.5 N.D. +1.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.6 +1.8 -0.2 N.D. +0.2
supernatant control Vogels_fungal_media 0.1X +0.3 +1.3 +0.5 N.D. +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.5 +0.8 +2.1 N.D. +0.7
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