Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS22465 and BPHYT_RS22470 overlap by 4 nucleotidesBPHYT_RS22470 and BPHYT_RS22475 are separated by 169 nucleotidesBPHYT_RS22475 and BPHYT_RS22480 are separated by 197 nucleotidesBPHYT_RS22480 and BPHYT_RS22485 are separated by 16 nucleotides BPHYT_RS22465: BPHYT_RS22465 - zinc carboxypeptidase-like protein, at 595,452 to 596,486 _RS22465 BPHYT_RS22470: BPHYT_RS22470 - hydrolase, at 596,483 to 597,283 _RS22470 BPHYT_RS22475: BPHYT_RS22475 - NAD-glutamate dehydrogenase, at 597,453 to 602,294 _RS22475 BPHYT_RS22480: BPHYT_RS22480 - cytochrome C551, at 602,492 to 603,397 _RS22480 BPHYT_RS22485: BPHYT_RS22485 - NADP-dependent aryl-alcohol dehydrogenase, at 603,414 to 604,394 _RS22485
Group Condition BPHYT_RS22465 BPHYT_RS22470 BPHYT_RS22475 BPHYT_RS22480 BPHYT_RS22485
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.3 -3.4 +0.1 +0.0 -1.5
supernatant control Vogels_fungal_media; pH 5.5 +0.1 -2.0 +0.0 -0.1 -0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.1 -1.9 +0.2 -0.6 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.4 -0.7 -0.7 -0.7 +0.7
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.1 -2.5 +0.4 +0.2 +0.1
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.1 -1.8 +0.1 +0.4 -0.1
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.6 -1.3 +0.2 +0.2 +0.0
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.6 -1.0 +0.1 +0.5 -0.2
phage P. phyto corn MOI 1 -0.1 -1.9 +0.2 +0.5 +0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.5 -1.0 +0.4 -0.3 +0.2
nitrogen source Ammonium chloride (N) +0.1 +0.6 +0.1 -0.5 -1.3
carbon source Sebacic (C) +0.6 -0.1 +0.1 -0.1 -1.4
nutrient Min media with glucose -0.3 -1.1 -0.1 +0.3 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.2 -1.7 +0.3 +0.1 +0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.7 -0.4 -0.3 +0.2 +1.1
nitrogen source L-Lysine (N) -0.3 +0.7 +0.2 -1.0 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.1 +0.7 +0.1 +0.5 -0.0
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.7 -1.6 -0.1 +0.0 +1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 +0.7 +0.3 -0.1 -0.7
phage 24hr PHYTO2 MOI 0.1 +0.4 -0.9 +0.3 +0.1 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.4 +1.8 -0.0 -0.4 -0.7
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 +0.5 +0.3 +0.7 +0.4
carbon source D-Glucosamine Hydrochloride (C) -0.1 +0.4 -0.0 -0.5 +1.6
carbon source Suberic (C) +1.2 +0.3 -0.1 +0.5 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.5 +0.8 -0.1 +0.1 +0.9
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +0.6 +0.4 +0.3 +0.5 +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 +1.2 +0.2 -0.2 +0.9
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 +2.4 -0.0 +0.4 +0.4
temperature LB 37C +0.5 -0.2 +0.1 +2.6 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.7 +1.5 +0.3 +0.2 +0.6
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