Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS22420 and BPHYT_RS22425 are separated by 32 nucleotidesBPHYT_RS22425 and BPHYT_RS22430 are separated by 221 nucleotidesBPHYT_RS22430 and BPHYT_RS22435 are separated by 10 nucleotidesBPHYT_RS22435 and BPHYT_RS22440 are separated by 123 nucleotides BPHYT_RS22420: BPHYT_RS22420 - membrane protein, at 585,029 to 585,217 _RS22420 BPHYT_RS22425: BPHYT_RS22425 - GntR family transcriptional regulator, at 585,250 to 586,665 _RS22425 BPHYT_RS22430: BPHYT_RS22430 - succinate-semialdehyde dehydrogenase, at 586,887 to 588,347 _RS22430 BPHYT_RS22435: BPHYT_RS22435 - 4-aminobutyrate aminotransferase, at 588,358 to 589,641 _RS22435 BPHYT_RS22440: BPHYT_RS22440 - DNA-binding protein, at 589,765 to 591,270 _RS22440
Group Condition BPHYT_RS22420 BPHYT_RS22425 BPHYT_RS22430 BPHYT_RS22435 BPHYT_RS22440
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -4.1 -0.5 +0.4 +0.2 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -3.9 -0.1 +0.0 +0.2 -0.3
nutrient Min media with glucose -1.9 -0.3 -0.0 -0.1 -0.1
carbon source Putrescine (C) +0.3 -0.4 -0.3 -1.7 -0.3
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -2.2 +0.2 +0.1 -0.1 -0.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -1.7 -0.4 +0.0 -0.2 -0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -2.9 +0.3 +0.4 -0.2 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -2.2 -0.2 +0.0 +0.1 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.1 -1.9 +0.2 -0.4 +0.1
nitrogen source L-Glutamic (N) -1.9 -0.4 +0.1 +0.5 +0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -1.7 +0.2 +0.2 +0.0 +0.0
nitrogen source L-Phenylalanine (N) -1.3 +0.5 -0.1 +0.1 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.5 -0.1 +0.1 +0.4 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.2 -0.4 +0.3 +0.1 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.1 -0.5 +0.1 +0.0 +0.4
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days -1.8 +0.4 +0.2 +0.2 +0.2
carbon source D,L-Lactate (C) -1.1 -0.3 +0.2 +0.5 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.9 -0.2 -0.2 +0.5 +0.4
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.5 +0.2 +0.1 -0.7 +0.7
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +1.2 -0.7 +0.2 -0.2 +0.3
supernatant control Vogels_fungal_media; pH 5.5 +0.6 +0.5 -0.0 -0.5 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.8 -0.7 +0.2 +1.2 +0.0
carbon source Protocatechuic Acid (C) +1.6 -0.2 +0.3 -0.1 +0.0
carbon source Protocatechuic Acid (C) +1.3 +0.4 +0.1 -0.1 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.6 +0.6 +0.7 -0.2 +0.2
carbon source Azelaic (C) +1.4 +0.7 +0.1 -0.4 +0.1
phage P. phyto PHYTO2 MOI 1 +0.4 +1.1 +0.3 +0.2 +0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.0 +0.2 +0.2 +0.2 -0.2
carbon source Sebacic (C) +1.6 +0.3 +0.4 +0.2 -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.6 +0.0 +0.1 +0.5 +0.5
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