Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS22370 and BPHYT_RS22375 overlap by 8 nucleotidesBPHYT_RS22375 and BPHYT_RS22380 are separated by 229 nucleotidesBPHYT_RS22380 and BPHYT_RS22385 are separated by 63 nucleotidesBPHYT_RS22385 and BPHYT_RS22390 are separated by 41 nucleotides BPHYT_RS22370: BPHYT_RS22370 - alpha/beta hydrolase, at 575,487 to 576,452 _RS22370 BPHYT_RS22375: BPHYT_RS22375 - 4-hydroxybenzoyl-CoA thioesterase, at 576,445 to 576,954 _RS22375 BPHYT_RS22380: BPHYT_RS22380 - Carnitine 3-dehydrogenase (EC 1.1.1.108) (from data), at 577,184 to 578,149 _RS22380 BPHYT_RS22385: BPHYT_RS22385 - hypothetical protein, at 578,213 to 579,142 _RS22385 BPHYT_RS22390: BPHYT_RS22390 - glycine/betaine ABC transporter substrate-binding protein, at 579,184 to 580,149 _RS22390
Group Condition BPHYT_RS22370 BPHYT_RS22375 BPHYT_RS22380 BPHYT_RS22385 BPHYT_RS22390
nitrogen source Carnitine Hydrochloride (N) +0.2 -3.9 -3.3 -3.5 -3.2
phage 36hr PHYTO1 MOI 1 -1.0 -0.1 -0.2 -0.1 +0.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.6 -0.1 -0.7 -0.6 +0.0
phage 24hr PHYTO6 MOI 10 +0.3 -0.4 -0.4 -0.3 +0.4
phage 24hr PHYTO6 MOI 0.1 -0.3 -0.2 -0.5 +0.1 +0.4
phage 24hr PHYTO2 MOI 1 -0.5 -0.3 -0.1 +0.4 +0.3
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.8 +0.0 -0.1 +0.4 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 +0.5 +0.5 -0.2 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 +0.3 +0.1 -0.4 -0.3
phage P. phyto PHYTO2 MOI 1 -0.7 -0.0 +0.5 +0.6 +0.1
phage PHYTO3 MOI 0.1 -0.5 -0.2 +0.3 +0.4 +0.4
motility outer cut, LB soft agar motility assay -0.2 +0.1 -0.4 +0.3 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 +0.3 -0.3 +0.0 +0.5
motility outer cut, LB soft agar motility assay +0.8 -0.3 -0.1 +0.1 +0.4
nitrogen source L-Isoleucine (N) +0.7 -0.0 -0.0 -0.3 +0.5
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.5 -0.1 +0.6 +0.2 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.2 +0.2 +0.5 +0.3 +0.4
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.0 +0.4 +0.2 +0.3 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.2 +0.7 +0.6 -0.4 +0.7
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.5 +0.2 -0.0 +0.6 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 +0.1 +0.0 +0.3 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.4 +0.2 +0.2 +0.6
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.2 +0.6 -0.0 +0.6 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days +0.3 -0.0 +0.4 +0.4 +0.4
r2a control R2A control +0.1 +0.5 +0.5 +0.3 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 +0.1 +0.4 +0.6 +0.2
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.0 +0.2 +0.5 -0.0 +1.0
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.4 +0.0 +0.2 +0.7 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 +0.4 +0.2 +0.5 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 +0.3 +0.2 +0.6 +0.4
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