Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS22335 and BPHYT_RS22340 are separated by 58 nucleotidesBPHYT_RS22340 and BPHYT_RS22345 are separated by 1692 nucleotidesBPHYT_RS22345 and BPHYT_RS22355 are separated by 1213 nucleotidesBPHYT_RS22355 and BPHYT_RS22360 are separated by 352 nucleotides BPHYT_RS22335: BPHYT_RS22335 - cytochrome C biogenesis protein, at 564,132 to 566,012 _RS22335 BPHYT_RS22340: BPHYT_RS22340 - methionine sulfoxide reductase A, at 566,071 to 566,802 _RS22340 BPHYT_RS22345: BPHYT_RS22345 - hypothetical protein, at 568,495 to 570,741 _RS22345 BPHYT_RS22355: BPHYT_RS22355 - HAD family hydrolase, at 571,955 to 572,629 _RS22355 BPHYT_RS22360: BPHYT_RS22360 - response regulator receiver, at 572,982 to 573,944 _RS22360
Group Condition BPHYT_RS22335 BPHYT_RS22340 BPHYT_RS22345 BPHYT_RS22355 BPHYT_RS22360
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.7 -0.4 -0.1 -2.3 -0.8
phage 24hr PHYTO2 MOI 1 +0.1 -0.4 +0.1 -2.5 -1.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.1 +0.6 +0.4 -2.0 -2.4
phage P. phyto corn MOI 1 -0.3 -0.2 +0.0 -1.8 -1.1
motility outer cut, LB soft agar motility assay -0.4 -0.4 -0.0 -0.7 -1.8
phage 24hr PHYTO MOI 0.1 -0.0 +0.0 -0.0 -1.1 -1.9
phage 24hr PHYTO6 MOI 0.1 +0.2 +0.3 -0.1 -1.4 -1.9
phage 24hr PHYTO6 MOI 1 +0.3 -0.2 +0.3 -1.5 -1.8
phage 24hr PHYTO10 MOI 10 +0.2 +0.4 +0.3 -2.0 -1.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days +0.1 -0.3 +0.6 -0.8 -2.3
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.4 +0.3 +0.1 -0.5 -2.2
phage 24hr PHYTO11 MOI 1 +0.2 -0.4 -0.3 -0.0 -2.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.3 +0.3 +0.1 -0.7 -2.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.1 -0.4 +0.3 -0.8 -1.5
phage 36hr PHYTO MOI 0.1 +0.1 +0.3 +0.3 -0.8 -2.0
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 +0.4 +0.0 -0.7 -1.7
carbon source Dodecandioic (C) -0.1 -0.6 -0.2 +0.6 -1.6
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.7 -0.2 +0.1 -1.2 -1.2
phage 24hr PHYTO9 MOI 10 -0.0 +0.9 +0.1 -0.3 -2.2
nophagecontrol 24hr Phyto Lib only +0.5 -0.2 +0.3 +0.3 -2.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.0 +1.0 +0.1 -0.2 -2.2
carbon source Tween 20 (C) +0.4 +0.2 -0.1 -2.2 +0.5
phage 36hr PHYTO9 MOI 0.1 +0.7 +0.2 +0.3 -0.6 -1.4
phage 24hr PHYTO6 MOI 10 -0.1 -0.2 +0.1 -1.7 +1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +1.1 +0.2 -0.0 -2.1
phage 24hr PHYTO9 MOI 0.1 +0.6 +0.4 +0.1 -2.9 +1.5
supernatant control Vogels_fungal_media; pH 5.5 +0.5 -0.1 -0.1 +1.9 -1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.2 +1.5 +0.1 +0.4 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.9 +0.7 +0.4 +0.4 -1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 +2.1 +0.2 -1.1 +0.5
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