Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS21800 and BPHYT_RS21805 are separated by 200 nucleotidesBPHYT_RS21805 and BPHYT_RS21810 overlap by 4 nucleotidesBPHYT_RS21810 and BPHYT_RS21815 are separated by 69 nucleotidesBPHYT_RS21815 and BPHYT_RS21820 are separated by 290 nucleotides BPHYT_RS21800: BPHYT_RS21800 - hypothetical protein, at 447,590 to 447,892 _RS21800 BPHYT_RS21805: BPHYT_RS21805 - transcriptional regulator, at 448,093 to 448,773 _RS21805 BPHYT_RS21810: BPHYT_RS21810 - histidine kinase, at 448,770 to 450,215 _RS21810 BPHYT_RS21815: BPHYT_RS21815 - hypothetical protein, at 450,285 to 450,557 _RS21815 BPHYT_RS21820: BPHYT_RS21820 - hypothetical protein, at 450,848 to 451,132 _RS21820
Group Condition BPHYT_RS21800 BPHYT_RS21805 BPHYT_RS21810 BPHYT_RS21815 BPHYT_RS21820
nophagecontrol 24hr Phyto Lib Only N.D. -5.0 -0.1 N.D. N.D.
phage 36hr PHYTO8 MOI 10 N.D. -2.5 -0.5 N.D. N.D.
phage 24hr PHYTO3 MOI 10 N.D. -2.1 -0.3 N.D. N.D.
phage 36hr PHYTO2 MOI 1 N.D. -2.0 -0.2 N.D. N.D.
phage 24hr PHYTO7 MOI 1 N.D. -1.4 -0.6 N.D. N.D.
phage 24hr PHYTO4 MOI 10 N.D. -1.4 -0.5 N.D. N.D.
carbon source Sebacic (C) N.D. -1.6 -0.3 N.D. N.D.
phage 36hr PHYTO11 MOI 1 N.D. -1.4 -0.5 N.D. N.D.
phage 24hr PHYTO11 MOI 1 N.D. -1.8 +0.0 N.D. N.D.
phage 36hr PHYTO11 MOI 0.1 N.D. -1.1 -0.5 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -1.1 -0.1 N.D. N.D.
nutrient casaminos N.D. -0.7 -0.5 N.D. N.D.
phage P. phyto corn MOI 1 N.D. -1.4 +0.3 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. +0.9 -1.7 N.D. N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X N.D. -0.8 +0.5 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.9 -0.6 N.D. N.D.
phage 36hr PHYTO2 MOI 1 N.D. +0.9 -0.5 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. +0.9 -0.5 N.D. N.D.
phage 36hr PHYTO10 MOI 10 N.D. +1.0 -0.5 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.9 -0.4 N.D. N.D.
carbon source L-Fucose (C) N.D. +0.9 -0.3 N.D. N.D.
carbon source Tween 20 (C) N.D. +1.1 -0.2 N.D. N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +1.1 -0.2 N.D. N.D.
carbon source D-Glucose (C) N.D. +1.2 -0.1 N.D. N.D.
pH LB, pH4 N.D. +1.0 +0.2 N.D. N.D.
pH LB, pH5 N.D. +1.1 +0.2 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +1.4 +0.0 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +1.2 +0.3 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. +1.3 +0.4 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +2.2 -0.5 N.D. N.D.
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