Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS21780 and BPHYT_RS21785 are separated by 439 nucleotidesBPHYT_RS21785 and BPHYT_RS21790 are separated by 88 nucleotidesBPHYT_RS21790 and BPHYT_RS21795 are separated by 407 nucleotidesBPHYT_RS21795 and BPHYT_RS21800 are separated by 39 nucleotides BPHYT_RS21780: BPHYT_RS21780 - MFS transporter, at 443,002 to 444,384 _RS21780 BPHYT_RS21785: BPHYT_RS21785 - hypothetical protein, at 444,824 to 445,156 _RS21785 BPHYT_RS21790: BPHYT_RS21790 - hypothetical protein, at 445,245 to 445,583 _RS21790 BPHYT_RS21795: BPHYT_RS21795 - membrane protein, at 445,991 to 447,550 _RS21795 BPHYT_RS21800: BPHYT_RS21800 - hypothetical protein, at 447,590 to 447,892 _RS21800
Group Condition BPHYT_RS21780 BPHYT_RS21785 BPHYT_RS21790 BPHYT_RS21795 BPHYT_RS21800
phage 36hr PHYTO10 MOI 10 -0.8 N.D. -1.8 N.D. N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -1.6 N.D. -0.7 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.8 N.D. -1.0 N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.5 N.D. -1.3 N.D. N.D.
phage 24hr PHYTO MOI 0.1 -1.0 N.D. -0.6 N.D. N.D.
supernatant control Vogels_fungal_media; pH 5.5 -1.0 N.D. -0.7 N.D. N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.0 N.D. -1.3 N.D. N.D.
nitrogen source L-Lysine (N) -0.8 N.D. -0.6 N.D. N.D.
phage 36hr PHYTO6 MOI 0.1 -0.9 N.D. -0.4 N.D. N.D.
phage 36hr PHYTO9 MOI 1 -1.0 N.D. -0.3 N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.1 N.D. -1.4 N.D. N.D.
phage 36hr PHYTO1 MOI 10 -0.9 N.D. -0.3 N.D. N.D.
phage 36hr PHYTO6 MOI 10 -0.8 N.D. -0.3 N.D. N.D.
phage P. phyto PHYTO2 MOI 0.1 -1.0 N.D. +0.2 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 N.D. -0.8 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.5 N.D. -0.7 N.D. N.D.
phage 36hr PHYTO6 MOI 1 -0.8 N.D. +0.7 N.D. N.D.
stress Lomefloxacin 0.001 mM -0.6 N.D. +0.6 N.D. N.D.
phage 36hr PHYTO8 MOI 10 -0.6 N.D. +0.7 N.D. N.D.
carbon source Phenylacetic 5 mM (C) -0.7 N.D. +0.9 N.D. N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.7 N.D. -0.5 N.D. N.D.
phage 24hr PHYTO3 MOI 10 +0.8 N.D. -0.4 N.D. N.D.
carbon source Phenylacetic 5 mM (C) +0.4 N.D. +0.8 N.D. N.D.
lb LB +0.5 N.D. +0.7 N.D. N.D.
stress Benzethonium chloride 0.0016 mM +0.6 N.D. +0.7 N.D. N.D.
phage 24hr PHYTO6 MOI 10 +0.9 N.D. +0.4 N.D. N.D.
carbon source D-Glucose (C) +1.2 N.D. +0.2 N.D. N.D.
stress Paraquat dichloride 0.032 mg/ml +0.4 N.D. +1.1 N.D. N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +1.0 N.D. +0.5 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.2 N.D. +0.3 N.D. N.D.
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BPHYT_RS21780
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BPHYT_RS21785
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