Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS21600 and BPHYT_RS21605 are separated by 106 nucleotidesBPHYT_RS21605 and BPHYT_RS21610 are separated by 188 nucleotidesBPHYT_RS21610 and BPHYT_RS21615 are separated by 117 nucleotidesBPHYT_RS21615 and BPHYT_RS21620 overlap by 4 nucleotides BPHYT_RS21600: BPHYT_RS21600 - 3-oxoacyl-ACP reductase, at 402,574 to 403,284 _RS21600 BPHYT_RS21605: BPHYT_RS21605 - NmrA family transcriptional regulator, at 403,391 to 404,143 _RS21605 BPHYT_RS21610: BPHYT_RS21610 - DNA-binding protein, at 404,332 to 405,861 _RS21610 BPHYT_RS21615: BPHYT_RS21615 - FMN-dependent NADH-azoreductase, at 405,979 to 406,653 _RS21615 BPHYT_RS21620: BPHYT_RS21620 - GCN5 family acetyltransferase, at 406,650 to 407,117 _RS21620
Group Condition BPHYT_RS21600 BPHYT_RS21605 BPHYT_RS21610 BPHYT_RS21615 BPHYT_RS21620
phage P. phyto PHYTO2 MOI 1 +0.3 N.D. +0.3 -2.7 -4.6
phage 24hr PHYTO7 MOI 1 -0.9 N.D. -0.2 -1.1 -2.2
phage 36hr PHYTO2 MOI 1 -0.2 N.D. +0.3 -1.1 -2.7
nophagecontrol 36hr Phyto Lib Only -0.4 N.D. -0.2 -0.3 -2.6
phage 36hr PHYTO6 MOI 10 -0.5 N.D. +0.0 -0.2 -2.6
phage 36hr PHYTO MOI 0.1 -0.6 N.D. +0.0 -0.3 -2.1
phage 36hr PHYTO2 MOI 1 -0.2 N.D. -0.2 -1.2 -1.4
nitrogen source L-Phenylalanine (N) -0.2 N.D. -0.3 +0.1 -2.4
phage 24hr PHYTO11 MOI 1 -0.9 N.D. +0.2 -0.5 -1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 N.D. -0.1 -0.4 -2.3
phage 36hr PHYTO4 MOI 0.1 -0.3 N.D. +0.1 -0.9 -1.2
phage 36hr PHYTO2 MOI 0.1 -0.3 N.D. -0.1 -0.3 -1.6
phage 24hr PHYTO9 MOI 0.1 -0.5 N.D. -0.0 -0.2 -1.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.2 N.D. -0.4 -0.3 -1.3
phage 24hr PHYTO3 MOI 10 -0.6 N.D. +0.1 +0.1 -1.8
phage 36hr PHYTO6 MOI 1 -0.0 N.D. +0.1 -0.7 -1.5
phage 36hr PHYTO3 MOI 1 +0.2 N.D. +0.0 -0.9 -1.4
phage 24hr PHYTO6 MOI 0.1 +0.3 N.D. +0.2 -0.9 -1.6
phage 36hr PHYTO8 MOI 10 +0.2 N.D. -0.1 -0.8 -1.3
nophagecontrol 24hr Phyto Lib only -0.1 N.D. +0.2 -1.1 -0.9
phage 24hr PHYTO6 MOI 0.1 -0.2 N.D. -0.1 +0.5 -2.1
phage 36hr PHYTO10 MOI 1 -0.2 N.D. +0.2 -0.7 -1.1
phage 24hr PHYTO1 MOI 10 -0.3 N.D. +0.5 -1.1 -0.9
phage 36hr PHYTO6 MOI 0.1 -0.4 N.D. +0.3 -0.4 -1.2
phage 36hr PHYTO1 MOI 10 +0.1 N.D. +0.2 -0.7 -1.3
phage 24hr PHYTO8 MOI 1 -0.1 N.D. +0.5 -0.7 -1.1
carbon source succinate (C) -0.9 N.D. -0.1 +0.6 -1.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 N.D. -0.4 -0.6 -0.6
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.5 N.D. +0.3 +1.4 -0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 N.D. +0.5 +0.7 +1.5
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BPHYT_RS21600
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BPHYT_RS21605
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BPHYT_RS21620
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