Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS21495 and BPHYT_RS21500 overlap by 4 nucleotidesBPHYT_RS21500 and BPHYT_RS21505 are separated by 78 nucleotidesBPHYT_RS21505 and BPHYT_RS21510 are separated by 31 nucleotidesBPHYT_RS21510 and BPHYT_RS21515 overlap by 4 nucleotides BPHYT_RS21495: BPHYT_RS21495 - ABC transporter, at 382,634 to 383,443 _RS21495 BPHYT_RS21500: BPHYT_RS21500 - ABC transporter, at 383,440 to 384,153 _RS21500 BPHYT_RS21505: BPHYT_RS21505 - amidohydrolase, at 384,232 to 385,431 _RS21505 BPHYT_RS21510: BPHYT_RS21510 - (2Fe-2S)-binding protein, at 385,463 to 385,981 _RS21510 BPHYT_RS21515: BPHYT_RS21515 - aldehyde dehydrogenase, at 385,978 to 389,601 _RS21515
Group Condition BPHYT_RS21495 BPHYT_RS21500 BPHYT_RS21505 BPHYT_RS21510 BPHYT_RS21515
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -4.8 N.D. +0.6 N.D. +0.0
carbon source Protocatechuic Acid (C) -0.6 N.D. -3.2 N.D. -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -3.4 N.D. +0.5 N.D. -0.3
carbon source Phenylacetic 5 mM (C) -3.6 N.D. +0.5 N.D. +0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.1 N.D. -0.3 N.D. -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.9 N.D. -0.5 N.D. -0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -1.8 N.D. -0.2 N.D. -0.4
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -2.3 N.D. +0.1 N.D. -0.0
motility inner cut, LB soft agar motility assay -1.4 N.D. -0.9 N.D. +0.0
phage 24hr PHYTO9 MOI 0.1 -1.3 N.D. -0.8 N.D. -0.0
nitrogen source Ammonium chloride (N) -1.9 N.D. -0.0 N.D. -0.1
nitrogen source Uridine (N) -1.2 N.D. -0.8 N.D. +0.0
carbon source Tween 20 (C) -2.4 N.D. +0.7 N.D. -0.1
nophagecontrol 36hr Phyto Lib only -1.3 N.D. -0.6 N.D. +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.5 N.D. +0.1 N.D. -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.8 N.D. -1.0 N.D. +0.2
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -2.2 N.D. +0.6 N.D. +0.0
carbon source Malonic (C) -1.7 N.D. +0.3 N.D. +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.4 N.D. -1.2 N.D. +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -2.2 N.D. +1.0 N.D. -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.1 N.D. +0.8 N.D. -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.7 N.D. -1.3 N.D. +0.3
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -1.1 N.D. +0.9 N.D. -0.0
phage 24hr PHYTO2 MOI 1 -1.0 N.D. +1.0 N.D. -0.1
phage 36hr PHYTO8 MOI 10 +1.1 N.D. -0.9 N.D. -0.0
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +1.5 N.D. -0.5 N.D. -0.1
carbon source D-Glucose (C) +0.9 N.D. +1.0 N.D. -0.1
lb_dap plate control LB_DAP agar plate, outgrowth in LB +1.4 N.D. +0.5 N.D. +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.8 N.D. +1.3 N.D. +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +5.7 N.D. +0.6 N.D. +0.0
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