Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS21300 and BPHYT_RS21305 are separated by 139 nucleotidesBPHYT_RS21305 and BPHYT_RS21310 are separated by 165 nucleotidesBPHYT_RS21310 and BPHYT_RS21315 are separated by 628 nucleotidesBPHYT_RS21315 and BPHYT_RS21320 are separated by 216 nucleotides BPHYT_RS21300: BPHYT_RS21300 - membrane protein, at 338,297 to 338,812 _RS21300 BPHYT_RS21305: BPHYT_RS21305 - hypothetical protein, at 338,952 to 339,455 _RS21305 BPHYT_RS21310: BPHYT_RS21310 - porin, at 339,621 to 340,772 _RS21310 BPHYT_RS21315: BPHYT_RS21315 - hypothetical protein, at 341,401 to 341,937 _RS21315 BPHYT_RS21320: BPHYT_RS21320 - alkaline phosphatase, at 342,154 to 344,106 _RS21320
Group Condition BPHYT_RS21300 BPHYT_RS21305 BPHYT_RS21310 BPHYT_RS21315 BPHYT_RS21320
phage PHYTO3 MOI 0.1 -0.9 -0.1 -0.0 -0.7 -0.1
phage 36hr PHYTO8 MOI 10 -1.0 +0.0 +0.0 -0.5 -0.3
carbon source Phenylacetic 5 mM (C) -0.4 -0.1 -0.2 -1.1 +0.1
phage 24hr PHYTO6 MOI 1 -0.7 +0.3 -0.5 -0.6 -0.1
motility inner cut, LB soft agar motility assay -0.9 -0.2 +0.1 -0.2 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -0.5 -0.3 +0.0 -0.3 -0.4
phage P. phyto PHYTO2 MOI 1 -0.5 -0.3 +0.1 -0.5 -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.4 -0.2 -0.2 -1.0 -0.1
phage 24hr PHYTO9 MOI 10 -0.6 +0.2 -0.2 -0.4 -0.1
carbon source Suberic (C) -0.4 +0.2 +0.2 -0.6 -0.3
nophagecontrol 24hr Phyto Lib Only -0.4 +0.3 -0.1 -0.8 -0.1
carbon source Sebacic (C) -0.5 +0.4 -0.1 -0.7 +0.0
phage 24hr PHYTO3 MOI 10 -0.4 +0.1 +0.3 -1.0 +0.0
phage 24hr PHYTO10 MOI 10 -0.5 +0.3 +0.1 -0.6 -0.1
phage 36hr PHYTO4 MOI 1 -1.0 +0.4 -0.3 -0.0 +0.1
phage 24hr PHYTO10 MOI 1 -0.8 -0.2 +0.0 +0.4 -0.3
phage 36hr PHYTO4 MOI 1 -0.8 -0.3 +0.1 +0.6 -0.1
phage 36hr PHYTO6 MOI 0.1 -0.3 +0.2 +0.2 -0.4 -0.2
phage 36hr PHYTO6 MOI 1 -0.8 +0.3 -0.2 +0.0 +0.3
phage 36hr PHYTO8 MOI 10 -0.2 +0.6 +0.1 -1.0 +0.0
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.4 -0.1 +0.3 -0.4 +0.2
phage P. phyto PHYTO2 MOI 0.1 -0.7 +0.2 -0.2 +0.2 +0.0
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.4 -0.2 -0.1 -0.7 +0.2
phage 36hr PHYTO10 MOI 10 -1.0 +0.2 -0.1 +0.6 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -0.3 +0.1 +0.9 -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 +0.0 +0.0 +0.9 -0.0
phage 24hr PHYTO3 MOI 10 +0.5 -0.2 -0.0 +0.3 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.8 -0.3 +0.1 +0.3 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +1.2 +0.1 -0.2 +0.2 -0.1
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.7 +0.2 +0.0 +0.2 +0.2
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