Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS21170 and BPHYT_RS21175 are separated by 125 nucleotidesBPHYT_RS21175 and BPHYT_RS21180 are separated by 891 nucleotidesBPHYT_RS21180 and BPHYT_RS21190 are separated by 1859 nucleotidesBPHYT_RS21190 and BPHYT_RS21195 are separated by 495 nucleotides BPHYT_RS21170: BPHYT_RS21170 - LysR family transcriptional regulator, at 309,881 to 310,831 _RS21170 BPHYT_RS21175: BPHYT_RS21175 - transposase, at 310,957 to 311,538 _RS21175 BPHYT_RS21180: BPHYT_RS21180 - hypothetical protein, at 312,430 to 313,455 _RS21180 BPHYT_RS21190: BPHYT_RS21190 - phospholipase C, at 315,315 to 317,000 _RS21190 BPHYT_RS21195: BPHYT_RS21195 - hypothetical protein, at 317,496 to 318,581 _RS21195
Group Condition BPHYT_RS21170 BPHYT_RS21175 BPHYT_RS21180 BPHYT_RS21190 BPHYT_RS21195
phage 36hr PHYTO4 MOI 0.1 -1.2 N.D. -1.3 +0.4 -0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.3 N.D. -0.1 -0.4 -0.9
carbon source 2-Deoxy-D-ribonic lithium salt (C) -1.7 N.D. -0.2 -0.0 -0.5
nophagecontrol 36hr Phyto Lib Only -2.5 N.D. -0.1 +0.4 -0.3
phage 36hr PHYTO6 MOI 10 -1.9 N.D. -0.5 +0.2 -0.2
phage 36hr PHYTO1 MOI 1 -1.5 N.D. -0.7 +0.2 -0.4
phage 24hr PHYTO2 MOI 0.1 -0.9 N.D. -0.5 +0.1 -1.0
nophagecontrol 36hr Phyto Lib only -1.5 N.D. -0.6 -0.4 +0.2
temperature LB, 37C -1.8 N.D. -0.2 +0.2 -0.6
phage 24hr PHYTO3 MOI 10 +0.7 N.D. -2.6 +0.6 -0.9
phage 36hr PHYTO1 MOI 10 -0.9 N.D. -0.6 +0.1 -0.8
phage 36hr PHYTO10 MOI 10 -1.5 N.D. -0.4 +0.4 -0.7
phage 36hr PHYTO6 MOI 0.1 -1.4 N.D. -0.5 +0.1 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.3 N.D. +0.2 -0.3 -0.6
phage 36hr PHYTO2 MOI 1 -0.8 N.D. -0.6 +0.6 -1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.7 N.D. -0.3 +0.3 -0.9
phage 24hr PHYTO2 MOI 1 -0.6 N.D. -0.5 +0.5 -1.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.8 N.D. -0.3 +0.6 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.6 N.D. -0.8 +0.5 -0.5
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -1.1 N.D. -0.3 -0.5 +0.4
phage 24hr PHYTO8 MOI 1 -1.1 N.D. +0.3 +0.2 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.9 N.D. -0.5 +0.7 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.8 N.D. -0.7 +0.8 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.9 N.D. -0.9 +1.0 -0.1
phage 36hr PHYTO8 MOI 10 -1.1 N.D. -0.4 +0.5 +0.4
phage 36hr PHYTO8 MOI 10 +0.9 N.D. -0.9 +0.2 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.7 N.D. -0.2 +1.1 -0.2
phage 36hr PHYTO9 MOI 0.1 +1.0 N.D. -0.9 +0.2 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 N.D. +1.7 -0.5 +0.3
supernatant control Vogels_fungal_media; pH 5.5 +1.2 N.D. -0.2 +0.4 +0.5
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