Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS21035 and BPHYT_RS21040 overlap by 4 nucleotidesBPHYT_RS21040 and BPHYT_RS21045 are separated by 178 nucleotidesBPHYT_RS21045 and BPHYT_RS21050 are separated by 523 nucleotidesBPHYT_RS21050 and BPHYT_RS21055 are separated by 2 nucleotides BPHYT_RS21035: BPHYT_RS21035 - ABC transporter permease, at 279,463 to 280,617 _RS21035 BPHYT_RS21040: BPHYT_RS21040 - ABC transporter permease, at 280,614 to 281,540 _RS21040 BPHYT_RS21045: BPHYT_RS21045 - bis(5'-nucleosyl)-tetraphosphatase, at 281,719 to 282,801 _RS21045 BPHYT_RS21050: BPHYT_RS21050 - aldehyde-activating protein, at 283,325 to 283,900 _RS21050 BPHYT_RS21055: BPHYT_RS21055 - S-formylglutathione hydrolase, at 283,903 to 284,733 _RS21055
Group Condition BPHYT_RS21035 BPHYT_RS21040 BPHYT_RS21045 BPHYT_RS21050 BPHYT_RS21055
phage 24hr PHYTO2 MOI 0.1 +0.2 N.D. -0.9 -0.2 -2.3
phage 36hr PHYTO2 MOI 1 -2.1 N.D. -1.5 -0.2 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.9 N.D. -0.8 -0.1 +0.4
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -1.7 N.D. -0.5 -1.1 +0.9
phage 24hr PHYTO4 MOI 10 -1.7 N.D. -0.0 -0.3 -0.3
phage 36hr PHYTO8 MOI 10 -0.2 N.D. -1.2 -0.1 -0.5
carbon source Suberic (C) -0.4 N.D. -0.9 -0.4 +0.1
nophagecontrol 24hr Phyto Lib Only +0.1 N.D. -2.0 +0.1 +0.2
carbon source L-Fucose (C) -0.1 N.D. +0.2 +0.2 -1.5
phage 36hr PHYTO3 MOI 1 +0.5 N.D. -0.1 -0.0 -1.4
nitrogen source L-Lysine (N) -0.2 N.D. -0.6 -0.6 +0.5
phage 36hr PHYTO8 MOI 10 +0.1 N.D. -0.5 -0.9 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.6 N.D. -0.4 -0.6 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.3 N.D. -0.9 +0.6 -0.4
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.7 N.D. +0.7 -0.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.1 N.D. +0.9 -0.1 -0.9
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.8 N.D. -1.1 +0.3 +0.5
supernatant control Vogels_fungal_media 0.1X +0.6 N.D. +0.3 -0.8 +0.4
motility outer cut, LB soft agar motility assay +0.9 N.D. -0.9 +0.5 +0.1
carbon source D-Glucose 20 mM (C) +1.0 N.D. -0.3 -0.3 +0.4
phage 24hr PHYTO6 MOI 10 +1.3 N.D. -0.2 -0.4 +0.1
phage 24hr PHYTO8 MOI 1 +0.7 N.D. -1.0 +0.0 +1.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.4 N.D. +0.3 +0.3 +0.9
phage 36hr PHYTO6 MOI 1 +0.1 N.D. +0.8 -0.3 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 N.D. +1.4 -0.2 -0.2
phage PHYTO3 MOI 0.1 -0.2 N.D. +0.1 +0.4 +1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.3 N.D. +0.6 -0.0 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 N.D. +0.5 +1.0 +0.4
phage 36hr PHYTO7 MOI 1 +0.5 N.D. +0.9 +0.0 +0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.5 N.D. +0.2 +0.4 +1.1
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