Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS21005 and BPHYT_RS21010 are separated by 58 nucleotidesBPHYT_RS21010 and BPHYT_RS21015 are separated by 202 nucleotidesBPHYT_RS21015 and BPHYT_RS21020 are separated by 272 nucleotidesBPHYT_RS21020 and BPHYT_RS21025 are separated by 329 nucleotides BPHYT_RS21005: BPHYT_RS21005 - asparaginase, at 270,764 to 271,756 _RS21005 BPHYT_RS21010: BPHYT_RS21010 - D-amino acid dehydrogenase, at 271,815 to 273,062 _RS21010 BPHYT_RS21015: BPHYT_RS21015 - transcriptional regulator, at 273,265 to 274,356 _RS21015 BPHYT_RS21020: BPHYT_RS21020 - branched-chain amino acid ABC transporter substrate-binding protein, at 274,629 to 275,753 _RS21020 BPHYT_RS21025: BPHYT_RS21025 - cation-binding protein, at 276,083 to 276,619 _RS21025
Group Condition BPHYT_RS21005 BPHYT_RS21010 BPHYT_RS21015 BPHYT_RS21020 BPHYT_RS21025
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.9 N.D. N.D. +0.1 -4.7
phage 24hr PHYTO6 MOI 10 -0.2 N.D. N.D. -0.5 -2.5
phage 36hr PHYTO11 MOI 0.1 -0.2 N.D. N.D. -0.4 -2.4
phage P. phyto PHYTO2 MOI 0.1 -0.0 N.D. N.D. -0.2 -2.4
nophagecontrol 36hr Phyto Lib only -0.0 N.D. N.D. -0.4 -2.3
phage 24hr PHYTO3 MOI 1 -0.5 N.D. N.D. -0.1 -1.7
phage 36hr PHYTO6 MOI 10 -0.4 N.D. N.D. +0.5 -2.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.1 N.D. N.D. +0.2 -2.0
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.3 N.D. N.D. -0.0 -1.8
nitrogen source NAG (N) +0.3 N.D. N.D. +0.2 -2.0
phage 36hr PHYTO9 MOI 1 -0.0 N.D. N.D. +0.4 -1.6
nitrogen source L-Citrulline (N) -0.3 N.D. N.D. +0.4 -1.3
supernatant control Vogels_fungal_media 0.1X +1.0 N.D. N.D. -0.1 -2.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.7 N.D. N.D. +0.5 -0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 N.D. N.D. +0.9 -1.2
r2a control R2A control -0.8 N.D. N.D. -0.7 +0.9
carbon source Tween 20 (C) +0.7 N.D. N.D. +0.3 -1.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 N.D. N.D. +0.9 -1.1
nitrogen source Putrescine (N) +0.4 N.D. N.D. +0.4 -1.2
supernatant control Vogels_fungal_media 0.1X +0.4 N.D. N.D. +0.5 -1.3
phage 36hr PHYTO10 MOI 10 -0.4 N.D. N.D. -0.2 +1.4
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.5 N.D. N.D. -0.2 +1.5
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.4 N.D. N.D. -0.5 +1.8
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +1.7 N.D. N.D. +0.4 -0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 N.D. N.D. -0.2 +1.5
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +1.6 N.D. N.D. +0.3 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 N.D. N.D. +0.4 +1.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.1 N.D. N.D. +0.7 +2.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +2.1 N.D. N.D. +0.2 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 N.D. N.D. +0.3 +4.6
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