Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS20880 and BPHYT_RS20885 are separated by 187 nucleotidesBPHYT_RS20885 and BPHYT_RS20890 are separated by 94 nucleotidesBPHYT_RS20890 and BPHYT_RS20895 are separated by 345 nucleotidesBPHYT_RS20895 and BPHYT_RS20900 are separated by 319 nucleotides BPHYT_RS20880: BPHYT_RS20880 - polyketide cyclase, at 245,946 to 246,491 _RS20880 BPHYT_RS20885: BPHYT_RS20885 - nuclear protein SET, at 246,679 to 247,119 _RS20885 BPHYT_RS20890: BPHYT_RS20890 - transglycosylase, at 247,214 to 247,465 _RS20890 BPHYT_RS20895: BPHYT_RS20895 - ABC transporter, at 247,811 to 249,604 _RS20895 BPHYT_RS20900: BPHYT_RS20900 - phytanoyl-CoA dioxygenase, at 249,924 to 251,138 _RS20900
Group Condition BPHYT_RS20880 BPHYT_RS20885 BPHYT_RS20890 BPHYT_RS20895 BPHYT_RS20900
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -4.2 +0.7 -0.1 -0.1 -2.0
nitrogen source L-Lysine (N) -4.3 -0.9 -0.5 -0.3 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -3.2 +0.1 -0.5 -0.0 -1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.5 -1.0 -0.4 -0.1 -1.7
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -4.4 -0.2 -0.1 +0.1 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.9 -0.4 -0.5 -0.4 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 -1.0 -0.1 +0.3 -2.4
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -2.3 -0.1 -0.1 -0.1 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.8 -1.1 +0.1 +0.4 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.9 -0.5 -0.2 +0.4 -1.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.5 +0.1 -0.5 +0.3 -0.9
lb_dap plate control LB_DAP agar plate, outgrowth in LB -2.2 +0.0 -0.3 -0.1 +0.2
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) -0.5 +0.2 -0.3 +0.3 -1.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.9 +0.6 +0.0 +0.5 -2.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.5 +1.3 -1.2 +0.1 +0.4
phage 24hr PHYTO3 MOI 10 -0.9 -0.3 -0.5 -0.6 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.6 -0.9 +0.6 -0.0 +0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -1.1 -0.9 -0.2 +0.2 +0.8
phage 36hr PHYTO1 MOI 1 +0.4 -0.1 +0.4 +0.1 -1.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.9 -0.7 -0.3 +0.2 +0.7
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.5 +0.4 -1.3 +0.2 -0.4
carbon source Protocatechuic Acid (C) +1.4 -0.1 +0.9 -0.0 -2.2
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.7 +0.1 +0.6 +1.0 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.3 -0.8 -0.2 +0.3 +1.4
motility outer cut, LB soft agar motility assay -1.6 +0.2 +1.2 +0.2 +0.5
phage 36hr PHYTO4 MOI 1 -0.8 +1.2 +0.8 +0.2 -0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.5 +0.5 +0.1 -0.2 -0.7
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.8 +0.9 +0.9 -0.1 +1.0
carbon source adipate (C) +1.6 +0.1 +1.1 -0.4 -0.3
phage P. phyto PHYTO2 MOI 1 +1.3 +0.6 -0.0 -0.4 +1.0
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