Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS20870 and BPHYT_RS20875 are separated by 523 nucleotidesBPHYT_RS20875 and BPHYT_RS20880 are separated by 197 nucleotidesBPHYT_RS20880 and BPHYT_RS20885 are separated by 187 nucleotidesBPHYT_RS20885 and BPHYT_RS20890 are separated by 94 nucleotides BPHYT_RS20870: BPHYT_RS20870 - cysteine hydrolase, at 244,374 to 244,943 _RS20870 BPHYT_RS20875: BPHYT_RS20875 - hypothetical protein, at 245,467 to 245,748 _RS20875 BPHYT_RS20880: BPHYT_RS20880 - polyketide cyclase, at 245,946 to 246,491 _RS20880 BPHYT_RS20885: BPHYT_RS20885 - nuclear protein SET, at 246,679 to 247,119 _RS20885 BPHYT_RS20890: BPHYT_RS20890 - transglycosylase, at 247,214 to 247,465 _RS20890
Group Condition BPHYT_RS20870 BPHYT_RS20875 BPHYT_RS20880 BPHYT_RS20885 BPHYT_RS20890
nitrogen source L-Lysine (N) +0.4 N.D. -4.3 -0.9 -0.5
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.2 N.D. -4.4 -0.2 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.0 N.D. -3.2 +0.1 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.4 N.D. -1.9 -0.4 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 N.D. -1.5 -1.0 -0.4
phage 24hr PHYTO2 MOI 0.1 -1.0 N.D. -2.1 -0.1 +0.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.7 N.D. -4.2 +0.7 -0.1
phage 24hr PHYTO10 MOI 10 -0.9 N.D. -0.8 -0.7 -0.2
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.1 N.D. -2.3 -0.1 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 N.D. -1.8 -1.1 +0.1
lb_dap plate control LB_DAP agar plate, outgrowth in LB -0.1 N.D. -2.2 +0.0 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 N.D. -1.3 -1.1 -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.3 N.D. -1.1 -0.9 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 N.D. -2.5 +1.3 -1.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.5 N.D. -1.5 +0.1 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 N.D. -1.6 -0.9 +0.6
phage 24hr PHYTO10 MOI 10 -0.6 N.D. -1.2 +0.4 +0.2
phage 36hr PHYTO2 MOI 1 -0.5 N.D. +0.7 -0.7 -0.7
motility outer cut, LB soft agar motility assay -0.0 N.D. -1.6 +0.2 +1.2
temperature LB, 37C -0.5 N.D. +0.5 -1.0 +0.9
carbon source D-Glucose (C) +0.5 N.D. +0.9 -0.6 -0.7
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.5 N.D. +1.5 -0.1 -0.4
phage 36hr PHYTO4 MOI 1 -0.1 N.D. -0.8 +1.2 +0.8
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.1 N.D. -0.8 +0.9 +0.9
phage 24hr PHYTO8 MOI 1 -0.8 N.D. +1.0 +0.7 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.6 N.D. +1.2 +0.7 -0.0
phage 24hr PHYTO2 MOI 1 -0.7 N.D. +0.7 +0.6 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.8 N.D. -0.2 +1.0 +0.5
motility outer cut, LB soft agar motility assay +0.4 N.D. +1.4 +0.3 +0.2
carbon source adipate (C) -0.1 N.D. +1.6 +0.1 +1.1
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BPHYT_RS20870
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