Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS20845 and BPHYT_RS20850 are separated by 351 nucleotidesBPHYT_RS20850 and BPHYT_RS20855 are separated by 126 nucleotidesBPHYT_RS20855 and BPHYT_RS20860 are separated by 204 nucleotidesBPHYT_RS20860 and BPHYT_RS20865 are separated by 33 nucleotides BPHYT_RS20845: BPHYT_RS20845 - peroxidase, at 239,273 to 239,929 _RS20845 BPHYT_RS20850: BPHYT_RS20850 - multidrug DMT transporter permease, at 240,281 to 241,123 _RS20850 BPHYT_RS20855: BPHYT_RS20855 - transcriptional regulator, at 241,250 to 242,158 _RS20855 BPHYT_RS20860: BPHYT_RS20860 - hypothetical protein, at 242,363 to 242,686 _RS20860 BPHYT_RS20865: BPHYT_RS20865 - 2-hydroxyacid dehydrogenase, at 242,720 to 243,685 _RS20865
Group Condition BPHYT_RS20845 BPHYT_RS20850 BPHYT_RS20855 BPHYT_RS20860 BPHYT_RS20865
phage P. phyto PHYTO2 MOI 1 -3.4 -0.5 +0.2 N.D. -1.1
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -1.4 -0.1 -0.4 N.D. -1.4
nophagecontrol 24hr Phyto Lib only -0.1 +0.1 +0.1 N.D. -3.1
nitrogen source L-Glutamic (N) -2.0 +0.2 +0.0 N.D. -0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.0 -0.4 -0.1 N.D. -1.1
phage 36hr PHYTO6 MOI 1 -1.4 -0.2 +0.3 N.D. -1.0
phage 24hr PHYTO9 MOI 10 -1.0 +0.6 -0.1 N.D. -1.3
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) -1.5 -0.2 -0.3 N.D. +0.3
phage 36hr PHYTO3 MOI 1 +0.4 -1.6 +0.2 N.D. -0.6
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -1.9 +0.6 +0.2 N.D. -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -2.3 +0.8 -0.4 N.D. +0.4
temperature LB, 37C +0.7 +0.1 -0.1 N.D. -2.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.4 -0.4 +0.4 N.D. -1.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.2 +0.3 +0.5 N.D. -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.2 +0.5 +0.7 N.D. +0.2
carbon source Phenylacetic 5 mM (C) -1.1 -0.2 -0.2 N.D. +0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.8 +0.9 -0.3 N.D. -0.5
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +1.0 -0.8 -0.2 N.D. -0.6
phage 36hr PHYTO1 MOI 1 +1.1 -0.3 +0.1 N.D. -0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 +1.3 -0.3 N.D. -0.4
nitrogen source L-Lysine (N) +1.1 +0.6 -0.5 N.D. -0.9
phage 36hr PHYTO1 MOI 10 -1.0 +0.9 +0.2 N.D. +0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 -0.5 +0.6 N.D. +1.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 +1.3 -0.5 N.D. -0.2
phage 36hr PHYTO8 MOI 10 +1.3 +0.3 +0.2 N.D. -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.6 +1.0 +0.3 N.D. -0.7
carbon source D-Glucose 20 mM (C) +0.9 +0.5 -0.4 N.D. +0.5
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.4 +0.3 +0.0 N.D. +1.9
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.5 +1.6 +0.1 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +2.4 +0.7 -0.0 N.D. -0.1
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