Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS20785 and BPHYT_RS20790 are separated by 130 nucleotidesBPHYT_RS20790 and BPHYT_RS20795 are separated by 246 nucleotidesBPHYT_RS20795 and BPHYT_RS20800 are separated by 95 nucleotidesBPHYT_RS20800 and BPHYT_RS20805 are separated by 126 nucleotides BPHYT_RS20785: BPHYT_RS20785 - CoA-transferase, at 224,526 to 226,013 _RS20785 BPHYT_RS20790: BPHYT_RS20790 - TetR family transcriptional regulator, at 226,144 to 226,794 _RS20790 BPHYT_RS20795: BPHYT_RS20795 - sorbosone dehydrogenase, at 227,041 to 228,549 _RS20795 BPHYT_RS20800: BPHYT_RS20800 - alanine-phosphoribitol ligase, at 228,645 to 230,318 _RS20800 BPHYT_RS20805: BPHYT_RS20805 - LacI family transcriptional regulator, at 230,445 to 231,524 _RS20805
Group Condition BPHYT_RS20785 BPHYT_RS20790 BPHYT_RS20795 BPHYT_RS20800 BPHYT_RS20805
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.3 -4.4 +0.1 -0.4 +0.2
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.5 -4.1 +0.2 +0.4 -0.0
carbon source 2-Deoxy-D-Ribose (C) -0.5 -3.2 -0.1 +0.1 +0.2
carbon source Malonic (C) -0.2 -2.5 -0.8 +0.1 +0.3
carbon source 2-Deoxy-D-Ribose (C) -0.1 -2.3 -0.5 -0.2 -0.1
carbon source 2-Deoxy-D-Ribose (C) +0.1 -2.7 -0.3 +0.1 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -2.4 -0.6 +0.4 -0.2
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.0 -3.2 +0.6 +0.5 -0.3
phage 24hr PHYTO11 MOI 1 +0.2 -2.2 -0.2 +0.1 -0.4
phage 24hr PHYTO9 MOI 10 +0.5 -2.3 +0.1 -0.4 -0.2
supernatant control Vogels_fungal_media 0.1X -0.2 -1.9 +0.1 -0.8 +0.6
carbon source L-Fucose (C) +0.0 -2.3 -0.2 +0.1 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X -1.1 -0.8 -0.0 -0.3 +0.6
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.4 -1.2 +0.5 +0.3 -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.0 -1.5 +0.6 +0.4 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 -1.9 +0.9 +0.4 -0.6
phage P. phyto PHYTO2 MOI 1 -0.2 -2.1 +0.5 -0.1 +0.5
phage 36hr PHYTO8 MOI 10 +0.4 -1.3 +0.3 +0.2 -0.7
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.6 -1.5 +0.5 +0.2 +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.8 -0.9 -0.3 +0.5 -0.7
phage 24hr PHYTO3 MOI 10 +0.2 -1.8 +0.7 +0.2 +0.3
supernatant control Vogels_fungal_media; pH 5.5 +0.6 -1.3 +0.5 +0.2 +0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 -0.9 +0.5 +0.2 +1.4
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.6 +1.2 -0.2 +0.4 +0.6
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.3 +1.8 -0.2 -0.5 +0.3
phage 36hr PHYTO8 MOI 10 -0.3 +1.7 +0.2 +0.4 -0.3
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.1 +2.1 -0.4 -0.0 +0.5
nophagecontrol 36hr Phyto Lib Only +0.8 +1.8 +0.1 +0.4 +0.3
phage 36hr PHYTO11 MOI 0.1 +0.8 +2.0 +0.3 -0.0 +1.0
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.3 +3.9 -0.2 +0.3 +4.6
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