Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS20735 and BPHYT_RS20740 are separated by 319 nucleotidesBPHYT_RS20740 and BPHYT_RS20745 are separated by 17 nucleotidesBPHYT_RS20745 and BPHYT_RS20750 are separated by 85 nucleotidesBPHYT_RS20750 and BPHYT_RS20755 are separated by 110 nucleotides BPHYT_RS20735: BPHYT_RS20735 - hypothetical protein, at 213,252 to 213,458 _RS20735 BPHYT_RS20740: BPHYT_RS20740 - D-ribose transporter ATP binding protein, at 213,778 to 215,289 _RS20740 BPHYT_RS20745: BPHYT_RS20745 - ribose ABC transporter permease, at 215,307 to 216,332 _RS20745 BPHYT_RS20750: BPHYT_RS20750 - LacI family transcriptional regulator, at 216,418 to 217,356 _RS20750 BPHYT_RS20755: BPHYT_RS20755 - fuconate dehydratase, at 217,467 to 218,765 _RS20755
Group Condition BPHYT_RS20735 BPHYT_RS20740 BPHYT_RS20745 BPHYT_RS20750 BPHYT_RS20755
phage 24hr PHYTO11 MOI 0.1 N.D. N.D. -0.9 -2.4 -0.7
phage P. phyto PHYTO2 MOI 1 N.D. N.D. -0.4 -2.3 -1.2
carbon source L-Arabinose (C) N.D. N.D. -0.2 -1.8 -0.6
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. -0.5 -1.6 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.5 -0.2 -1.4
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. N.D. -0.6 -1.3 +0.2
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. N.D. -0.6 -1.2 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.1 -1.6 -0.1
carbon source Suberic (C) N.D. N.D. +0.1 -2.2 +0.5
carbon source Phenylacetic 5 mM (C) N.D. N.D. -0.3 -1.3 +0.4
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.4 -1.1 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. +0.8 -2.3 +0.4
phage 36hr PHYTO2 MOI 1 N.D. N.D. -1.7 +0.5 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.5 -1.1 +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. +0.6 -1.5 +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +0.5 -1.3 +0.0
vitamin With vitamins t1 N.D. N.D. +0.1 -1.3 +0.6
nitrogen source L-Lysine (N) N.D. N.D. +0.5 -1.2 +0.3
phage 36hr PHYTO2 MOI 1 N.D. N.D. -1.0 +1.0 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. N.D. +0.7 -0.9 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +1.2 -1.0 +0.0
phage 36hr PHYTO7 MOI 0.1 N.D. N.D. -0.1 +1.0 -0.7
phage 24hr PHYTO7 MOI 1 N.D. N.D. -0.7 +0.8 +0.4
phage 24hr PHYTO3 MOI 10 N.D. N.D. -0.2 +1.6 -0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. N.D. +0.7 -0.6 +0.7
nophagecontrol 36hr Phyto Lib only N.D. N.D. +0.5 +1.3 -0.7
phage 24hr PHYTO4 MOI 0.1 N.D. N.D. -0.5 +1.2 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. +0.9 -0.7 +0.9
carbon source Dodecandioic (C) N.D. N.D. +0.4 +1.1 +0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.2 +2.8 +1.3
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