Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS20580 and BPHYT_RS20585 are separated by 229 nucleotidesBPHYT_RS20585 and BPHYT_RS20590 are separated by 132 nucleotidesBPHYT_RS20590 and BPHYT_RS20595 are separated by 162 nucleotidesBPHYT_RS20595 and BPHYT_RS20600 are separated by 85 nucleotides BPHYT_RS20580: BPHYT_RS20580 - NADH dehydrogenase, at 177,919 to 179,145 _RS20580 BPHYT_RS20585: BPHYT_RS20585 - MarR family transcriptional regulator, at 179,375 to 179,836 _RS20585 BPHYT_RS20590: BPHYT_RS20590 - methionine sulfoxide reductase A, at 179,969 to 180,475 _RS20590 BPHYT_RS20595: BPHYT_RS20595 - hypothetical protein, at 180,638 to 180,910 _RS20595 BPHYT_RS20600: BPHYT_RS20600 - peroxiredoxin, at 180,996 to 181,421 _RS20600
Group Condition BPHYT_RS20580 BPHYT_RS20585 BPHYT_RS20590 BPHYT_RS20595 BPHYT_RS20600
phage P. phyto PHYTO2 MOI 1 +0.6 N.D. -3.4 -0.2 -0.7
phage 24hr PHYTO10 MOI 10 -0.0 N.D. -2.2 -1.3 +0.3
nophagecontrol 36hr Phyto Lib Only +0.2 N.D. -2.3 -0.8 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.3 N.D. -1.8 -0.5 -0.4
phage 36hr PHYTO8 MOI 0.1 +0.1 N.D. -1.6 -0.7 -0.1
phage 24hr PHYTO11 MOI 1 -0.0 N.D. -1.5 -1.0 +0.2
phage P. phyto corn MOI 0.1 +0.0 N.D. -1.6 -0.5 -0.3
phage 36hr PHYTO6 MOI 0.1 -0.4 N.D. -1.3 -0.7 +0.3
nitrogen source L-Serine (N) -0.4 N.D. -0.8 -1.0 +0.1
phage 24hr PHYTO MOI 0.1 -0.1 N.D. -1.9 -0.1 +0.1
phage 36hr PHYTO2 MOI 1 +0.2 N.D. -2.1 -0.3 +0.7
phage 24hr PHYTO4 MOI 10 +0.4 N.D. -1.2 -1.0 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.4 N.D. -0.9 -1.2 +0.4
motility inner cut, LB soft agar motility assay +0.4 N.D. -1.1 -0.6 +0.3
phage 24hr PHYTO9 MOI 0.1 +0.6 N.D. -1.2 -0.6 +0.2
phage 36hr PHYTO10 MOI 10 -0.0 N.D. -0.4 -1.2 +0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.8 N.D. -0.7 -0.5 -0.4
carbon source adipate (C) +0.4 N.D. -1.7 +0.1 +0.5
carbon source Protocatechuic Acid (C) +0.2 N.D. -1.4 +0.2 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 N.D. -0.9 +0.6 -0.5
supernatant control Vogels_fungal_media 0.1X +0.2 N.D. +0.9 -0.9 +0.3
phage 24hr PHYTO6 MOI 10 +0.1 N.D. -0.9 +0.7 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 N.D. -1.0 +0.7 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +1.0 N.D. -0.3 -0.3 +0.6
carbon source Phenylacetic 5 mM (C) -0.6 N.D. +1.0 +0.2 +0.6
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +0.6 N.D. +0.7 -0.5 +1.0
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.4 N.D. +1.9 -0.1 +0.1
carbon source D-Glucose 20 mM (C) +0.0 N.D. +0.6 +0.9 +0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 N.D. -0.1 +1.3 +1.0
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.7 N.D. +1.7 +0.7 +0.6
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