Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS20445 and BPHYT_RS20450 are separated by 51 nucleotidesBPHYT_RS20450 and BPHYT_RS20455 are separated by 108 nucleotidesBPHYT_RS20455 and BPHYT_RS20460 are separated by 227 nucleotidesBPHYT_RS20460 and BPHYT_RS20465 are separated by 116 nucleotides BPHYT_RS20445: BPHYT_RS20445 - NAD-dependent dehydratase, at 149,568 to 150,632 _RS20445 BPHYT_RS20450: BPHYT_RS20450 - 6-chlorohydroxyquinol-1,2-dioxygenase, at 150,684 to 151,547 _RS20450 BPHYT_RS20455: BPHYT_RS20455 - hypothetical protein, at 151,656 to 152,333 _RS20455 BPHYT_RS20460: BPHYT_RS20460 - NmrA family transcriptional regulator, at 152,561 to 153,442 _RS20460 BPHYT_RS20465: BPHYT_RS20465 - LysR family transcriptional regulator, at 153,559 to 154,443 _RS20465
Group Condition BPHYT_RS20445 BPHYT_RS20450 BPHYT_RS20455 BPHYT_RS20460 BPHYT_RS20465
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.4 -0.5 -0.4 +0.1 -0.7
nitrogen source L-Glutamic (N) -0.0 -0.3 -0.3 -0.5 -0.7
carbon source Sebacic (C) -0.2 -0.1 +0.2 -0.1 -1.1
carbon source Phenylacetic 5 mM (C) -0.5 +0.1 -0.2 +0.2 -0.6
phage 36hr PHYTO3 MOI 1 +0.1 +0.5 +0.0 -0.1 -1.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.3 -0.5 -0.2 +0.2 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.5 -0.6 -0.1 +0.1 +0.5
phage 36hr PHYTO2 MOI 1 -0.1 -0.2 +0.1 +0.5 -0.7
nophagecontrol 36hr Phyto Lib only +0.5 +0.1 -0.2 -0.0 -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 -0.4 -0.0 +0.4 -0.6
nitrogen source L-Citrulline (N) -0.2 +0.6 +0.3 -0.3 -0.5
supernatant control Vogels_fungal_media 0.1X -0.2 -0.6 +0.1 -0.2 +1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.2 -0.1 -0.6 +0.0 +0.7
phage 24hr PHYTO4 MOI 10 +0.6 +0.2 +0.4 -0.1 -0.9
phage 36hr PHYTO8 MOI 10 +0.8 -0.7 +0.1 +0.2 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days -0.2 -0.7 +0.5 +0.4 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.1 -0.5 +0.1 -0.4 +1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.5 -0.5 +0.1 +0.2 +0.4
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.2 +0.8 +0.2 +0.2 -0.7
vitamin No vitamins t2 +0.3 +0.5 -0.2 +0.4 -0.3
motility inner cut, LB soft agar motility assay -0.2 -0.2 +0.6 -0.3 +0.9
supernatant control Vogels_fungal_media 0.1X +0.2 +0.1 -0.6 +0.1 +1.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 +0.5 +0.5 -0.3 -0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 +0.3 +0.4 -0.0 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.4 -0.4 +0.2 +0.1 +0.8
carbon source Malonic (C) +0.5 +0.4 +0.3 +0.2 -0.3
lb_dap plate control LB_DAP agar plate, outgrowth in LB +0.5 +0.1 -0.2 +0.1 +0.7
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.3 +0.4 -0.1 -0.1 +0.9
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.4 +0.5 +0.4 +0.3 +0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +0.0 +0.2 +0.3 +1.6
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