Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS20400 and BPHYT_RS20405 are separated by 423 nucleotidesBPHYT_RS20405 and BPHYT_RS20410 are separated by 132 nucleotidesBPHYT_RS20410 and BPHYT_RS20415 are separated by 76 nucleotidesBPHYT_RS20415 and BPHYT_RS20420 are separated by 147 nucleotides BPHYT_RS20400: BPHYT_RS20400 - GntR family transcriptional regulator, at 139,047 to 139,757 _RS20400 BPHYT_RS20405: BPHYT_RS20405 - MFS transporter, at 140,181 to 141,512 _RS20405 BPHYT_RS20410: BPHYT_RS20410 - glucarate dehydratase, at 141,645 to 142,994 _RS20410 BPHYT_RS20415: BPHYT_RS20415 - 5-dehydro-4-deoxyglucarate dehydratase, at 143,071 to 143,988 _RS20415 BPHYT_RS20420: BPHYT_RS20420 - DSBA oxidoreductase, at 144,136 to 145,644 _RS20420
Group Condition BPHYT_RS20400 BPHYT_RS20405 BPHYT_RS20410 BPHYT_RS20415 BPHYT_RS20420
phage P. phyto PHYTO2 MOI 1 N.D. +0.0 -1.4 -0.5 +0.3
phage 36hr PHYTO1 MOI 10 N.D. -0.2 -0.5 -0.7 +0.0
phage P. phyto corn MOI 0.1 N.D. -0.1 -0.9 -0.4 +0.1
carbon source Phenylacetic 5 mM (C) N.D. -0.6 -0.4 -0.2 -0.1
phage 24hr PHYTO6 MOI 0.1 N.D. +0.0 -1.0 -0.3 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.4 -0.8 +0.1 -0.1
carbon source Protocatechuic Acid (C) N.D. +0.1 -0.6 -0.2 -0.5
supernatant control Vogels_fungal_media 0.1X N.D. -0.7 -0.5 +0.3 -0.1
phage 36hr PHYTO3 MOI 1 N.D. -0.2 -1.1 +0.1 +0.2
carbon source D-Glucose (C) N.D. +0.1 -0.8 -0.5 +0.3
carbon source D-Glucose (C) N.D. +0.1 +0.3 -0.6 -0.4
phage 36hr PHYTO2 MOI 1 N.D. +0.2 -0.4 -0.6 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.1 -0.9 +0.2 +0.3
nitrogen source L-Isoleucine (N) N.D. -0.2 +0.6 -0.4 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.2 -0.4 -0.5 +0.5
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.3 -0.3 -0.4 +0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.2 +0.5 -0.5 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.1 -0.5 +0.1 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.3 -0.6 +0.7 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.3 -0.3 +0.2 +0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.4 -0.5 +0.1 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.1 -0.6 +0.5 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.3 -0.5 +0.2 +0.8
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.2 +0.8 +0.5 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.4 -0.2 -0.1 +0.8
temperature LB 37C N.D. +0.0 -0.2 +1.2 -0.0
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. -0.2 +0.6 +0.5 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.0 +0.7 -0.2 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.2 +0.9 -0.1 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.1 +0.3 +0.9 +0.0
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