Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS20100 and BPHYT_RS20105 are separated by 155 nucleotidesBPHYT_RS20105 and BPHYT_RS20110 are separated by 66 nucleotidesBPHYT_RS20110 and BPHYT_RS20115 are separated by 25 nucleotidesBPHYT_RS20115 and BPHYT_RS20120 are separated by 182 nucleotides BPHYT_RS20100: BPHYT_RS20100 - sensor histidine kinase, at 51,829 to 53,223 _RS20100 BPHYT_RS20105: BPHYT_RS20105 - nitrate ABC transporter substrate-binding protein, at 53,379 to 54,419 _RS20105 BPHYT_RS20110: BPHYT_RS20110 - sensor histidine kinase, at 54,486 to 55,868 _RS20110 BPHYT_RS20115: BPHYT_RS20115 - transcriptional regulator, at 55,894 to 56,592 _RS20115 BPHYT_RS20120: BPHYT_RS20120 - porin, at 56,775 to 57,899 _RS20120
Group Condition BPHYT_RS20100 BPHYT_RS20105 BPHYT_RS20110 BPHYT_RS20115 BPHYT_RS20120
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.8 -2.0 +0.3 -1.0 -0.3
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -0.0 -0.6 +0.1 -1.3 -1.2
phage 36hr PHYTO7 MOI 0.1 +0.2 -1.8 -0.2 +0.7 -0.8
phage P. phyto PHYTO2 MOI 1 -0.5 +0.3 +0.5 +0.7 -2.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 -0.3 -0.8 -0.6 +0.9
nophagecontrol 24hr Phyto Lib only +0.2 +0.6 +0.1 -0.8 -1.0
phage 24hr PHYTO6 MOI 1 +0.2 -0.6 -0.5 +1.2 -0.6
phage 36hr PHYTO11 MOI 1 -0.4 +0.9 +0.0 +0.7 -1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.0 +0.4 +0.2 -0.6 +1.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 +1.0 -0.4 +0.1 +0.5
carbon source Phenylacetic 5 mM (C) -0.4 +0.7 -1.0 +0.1 +1.0
phage 36hr PHYTO10 MOI 1 -0.2 -1.0 -0.2 +1.5 +0.3
motility outer cut, LB soft agar motility assay -0.8 +0.1 +0.4 +1.2 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.9 +0.3 -0.3 +0.4 +1.0
phage 36hr PHYTO1 MOI 1 -0.2 +0.3 +0.3 +1.1 -1.0
phage 24hr PHYTO1 MOI 10 -0.0 +0.3 -0.2 +1.5 -0.9
phage 36hr PHYTO2 MOI 1 -0.1 +1.0 +0.5 +0.6 -1.4
phage 36hr PHYTO9 MOI 0.1 -0.4 +0.8 +0.2 +1.0 -0.8
nophagecontrol 36hr Phyto Lib only -0.1 +0.9 +0.0 +1.0 -1.0
phage 36hr PHYTO MOI 0.1 -0.1 +0.7 +0.3 +0.8 -0.8
phage 24hr PHYTO3 MOI 0.1 -0.4 +0.9 +0.1 +1.1 -0.6
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.6 -0.2 -0.7 +1.5 -0.0
nophagecontrol 36hr Phyto Lib Only +0.4 +0.3 +0.4 +1.1 -0.9
phage 36hr PHYTO11 MOI 0.1 +0.2 +0.6 +0.0 +1.5 -0.8
phage 36hr PHYTO9 MOI 1 +0.4 +0.5 +0.3 +0.9 -0.6
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.0 -0.4 -0.1 +1.2 +1.0
phage 36hr PHYTO11 MOI 1 +0.3 +0.8 -0.1 +1.2 -0.5
phage 24hr PHYTO4 MOI 0.1 +0.2 +0.5 +0.2 +1.5 -0.4
phage 24hr PHYTO6 MOI 10 +0.3 +0.3 +0.7 +1.5 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.0 +2.8 +0.7 -0.0 +0.3
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