Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS20050 and BPHYT_RS20055 are separated by 342 nucleotidesBPHYT_RS20055 and BPHYT_RS20060 are separated by 227 nucleotidesBPHYT_RS20060 and BPHYT_RS20065 overlap by 1 nucleotidesBPHYT_RS20065 and BPHYT_RS20070 are separated by 81 nucleotides BPHYT_RS20050: BPHYT_RS20050 - hypothetical protein, at 43,294 to 43,542 _RS20050 BPHYT_RS20055: BPHYT_RS20055 - hypothetical protein, at 43,885 to 44,202 _RS20055 BPHYT_RS20060: BPHYT_RS20060 - hypothetical protein, at 44,430 to 45,122 _RS20060 BPHYT_RS20065: BPHYT_RS20065 - hypothetical protein, at 45,122 to 45,604 _RS20065 BPHYT_RS20070: BPHYT_RS20070 - hypothetical protein, at 45,686 to 47,119 _RS20070
Group Condition BPHYT_RS20050 BPHYT_RS20055 BPHYT_RS20060 BPHYT_RS20065 BPHYT_RS20070
carbon source adipate (C) N.D. -0.6 -0.9 -0.6 -1.8
motility inner cut, LB soft agar motility assay N.D. -1.0 -1.9 -0.7 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.4 -2.3 -0.1 -0.9
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.0 -2.1 -0.2 -1.3
carbon source Suberic (C) N.D. -0.8 +0.1 -0.6 -2.3
carbon source Sebacic (C) N.D. -0.6 +0.3 -0.4 -2.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.4 -2.0 -0.4 -1.0
carbon source succinate (C) N.D. -0.1 -0.1 +0.4 -3.1
carbon source Dodecandioic (C) N.D. +0.2 -0.0 +0.0 -3.1
phage 24hr PHYTO11 MOI 1 N.D. +0.6 -1.4 -1.1 -0.9
carbon source Phenylacetic 5 mM (C) N.D. +0.2 +0.4 -0.2 -3.1
carbon source Phenylacetic 5 mM (C) N.D. +0.6 -0.5 +0.2 -2.6
carbon source D-Glucose 20 mM (C) N.D. +0.7 -0.4 +0.1 -2.6
phage 36hr PHYTO1 MOI 10 N.D. +0.9 -1.4 -0.6 -0.9
phage 24hr PHYTO10 MOI 10 N.D. +1.0 -4.0 -0.1 +1.2
phage 24hr PHYTO7 MOI 10 N.D. +1.5 -0.4 -0.8 -1.7
phage 36hr PHYTO6 MOI 1 N.D. +1.2 -2.4 -0.4 +0.2
carbon source D-Glucose 20 mM (C) N.D. +0.7 +0.8 +0.1 -2.8
phage 24hr PHYTO1 MOI 10 N.D. +0.8 -0.5 -1.7 +0.4
phage 24hr PHYTO1 MOI 1 N.D. +0.6 -1.8 -0.3 +0.8
phage 24hr PHYTO1 MOI 10 N.D. +0.5 -1.4 -0.7 +0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.7 -1.1 -0.7 +1.0
phage 36hr PHYTO2 MOI 1 N.D. +0.8 +1.4 -0.7 -1.6
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. +0.2 +0.6 +1.1 -1.7
phage 36hr PHYTO10 MOI 1 N.D. +1.3 +0.4 -1.8 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.7 -1.9 -0.5 +2.4
phage 36hr PHYTO6 MOI 10 N.D. +1.2 +1.3 -0.6 +0.4
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. +0.8 +1.9 +0.3 -0.6
phage 36hr PHYTO11 MOI 0.1 N.D. +0.6 +0.9 -0.2 +1.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. +1.2 +0.2 +0.3 +2.3
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