Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS19810 and BPHYT_RS19820 are separated by 1300 nucleotidesBPHYT_RS19820 and BPHYT_RS19825 are separated by 484 nucleotidesBPHYT_RS19825 and BPHYT_RS19830 are separated by 174 nucleotidesBPHYT_RS19830 and BPHYT_RS19835 are separated by 370 nucleotides BPHYT_RS19810: BPHYT_RS19810 - chemotaxis protein, at 4,457,340 to 4,458,965 _RS19810 BPHYT_RS19820: BPHYT_RS19820 - hypothetical protein, at 4,460,266 to 4,460,589 _RS19820 BPHYT_RS19825: BPHYT_RS19825 - integrase, at 4,461,074 to 4,462,288 _RS19825 BPHYT_RS19830: BPHYT_RS19830 - tRNA modification GTPase TrmE, at 4,462,463 to 4,463,857 _RS19830 BPHYT_RS19835: BPHYT_RS19835 - XRE family transcriptional regulator, at 4,464,228 to 4,464,635 _RS19835
Group Condition BPHYT_RS19810 BPHYT_RS19820 BPHYT_RS19825 BPHYT_RS19830 BPHYT_RS19835
phage P. phyto PHYTO2 MOI 1 -0.5 -0.2 -0.6 N.D. -0.1
carbon source Sebacic (C) -0.7 -0.2 +0.1 N.D. -0.5
carbon source Protocatechuic Acid (C) -0.2 -0.0 -0.3 N.D. -0.7
phage P. phyto PHYTO2 MOI 1 -0.6 -0.3 +0.3 N.D. -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.7 +0.1 -0.1 N.D. -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -0.4 -0.3 N.D. -0.5
carbon source Phenylacetic 5 mM (C) -0.1 +0.2 -0.5 N.D. -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 -0.4 -0.2 N.D. -0.4
nitrogen source L-Lysine (N) -0.1 -0.2 -0.7 N.D. +0.2
phage 24hr PHYTO11 MOI 0.1 -0.1 +0.1 +0.2 N.D. -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.1 -0.2 +0.4 N.D. -0.7
phage 24hr PHYTO9 MOI 10 -0.6 -0.2 +0.6 N.D. -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 -0.3 +0.7 N.D. -1.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +0.5 -0.3 N.D. -0.2
carbon source Suberic (C) -0.2 +0.1 +0.3 N.D. -0.6
phage 24hr PHYTO7 MOI 10 -0.3 +0.1 +0.4 N.D. -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.6 -0.1 -0.2 N.D. -0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -0.0 +0.6 N.D. -0.5
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.4 -0.3 +0.7 N.D. -0.0
phage 36hr PHYTO9 MOI 1 -0.2 -0.3 +0.2 N.D. +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 -0.0 +0.4 N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.2 +0.2 +0.4 N.D. -0.5
carbon source Tween 20 (C) +0.4 -0.3 +0.4 N.D. -0.2
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.6 -0.3 +0.5 N.D. -0.4
motility outer cut, LB soft agar motility assay +0.5 +0.4 +0.2 N.D. -0.2
carbon source adipate (C) +0.2 -0.1 -0.2 N.D. +1.0
phage 36hr PHYTO10 MOI 10 -0.1 +0.3 +0.6 N.D. +0.2
phage 24hr PHYTO4 MOI 10 +0.1 +0.1 +0.5 N.D. +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.5 -0.1 +0.3 N.D. +0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -0.2 +1.1 N.D. +0.3
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